Hussein H A, Parwani A V, Rosen B I, Lucchelli A, Saif L J
Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
J Clin Microbiol. 1993 Sep;31(9):2491-6. doi: 10.1128/jcm.31.9.2491-2496.1993.
On the basis of antigenic variability in the VP7 outer capsid glycoprotein, at least 14 G serotypes exist for group A rotaviruses. Serotypic diversity exists among bovine rotaviruses (BRV), with serotypes G1, G6, G8, and G10 reported for cattle. Although G1 and G8 rotaviruses were originally described for humans, the recent isolation of G6 and G10 rotaviruses from humans further emphasizes the serotypic similarity between human and bovine rotaviruses and the possible zoonotic potential of rotaviruses. Results of our previous studies have indicated that more than 24% of BRV-positive field samples from diarrheic calves were nonreactive with cDNA probes or monoclonal antibodies to serotypes G6, G8, and G10. In this study, cDNA probes were prepared by polymerase chain reaction amplification of the hyperdivergent regions of the VP7 genes (nucleotides 51 to 392) from human (G1, G2, and G3) and porcine (G4, G5, and G11) rotaviruses. These probes were used in a dot blot hybridization assay to further characterize the G types of 59 BRV strains (fecal samples from diarrheic calves in Ohio, Nebraska, Washington, and South Dakota) that were nonreactive with cDNA probes to G6, G8, and G10. Rotaviruses belonging to serotypes G1 (n = 7), G2 (n = 1), G3 (n = 2), and G11 (n = 3) were identified among the BRV field samples. The BRV associated with these G types accounted for 22% of the samples tested; the other 78% of these samples remained untypeable with these probes. To our knowledge, this is the first report in the United States of the identification among BRV isolates of rotavirus serotypes G1, G2, G3, and G11.
基于A组轮状病毒VP7外衣壳糖蛋白的抗原变异性，至少存在14种G血清型。牛轮状病毒（BRV）存在血清型多样性，已报道牛的血清型有G1、G6、G8和G10。尽管G1和G8轮状病毒最初是在人类中描述的，但最近从人类中分离出G6和G10轮状病毒进一步强调了人类和牛轮状病毒之间的血清型相似性以及轮状病毒可能的人畜共患病潜力。我们之前的研究结果表明，来自腹泻犊牛的BRV阳性现场样本中，超过24%与针对G6、G8和G10血清型的cDNA探针或单克隆抗体无反应。在本研究中，通过聚合酶链反应扩增来自人类（G1、G2和G3）和猪（G4、G5和G11）轮状病毒的VP7基因高变区（核苷酸51至392）制备cDNA探针。这些探针用于斑点印迹杂交试验，以进一步鉴定59株BRV毒株（来自俄亥俄州、内布拉斯加州、华盛顿州和南达科他州腹泻犊牛的粪便样本）的G型，这些毒株与针对G6、G8和G10的cDNA探针无反应。在BRV现场样本中鉴定出属于G1（n = 7）、G2（n = 1）、G3（n = 2）和G11（n = 3）血清型的轮状病毒。与这些G型相关的BRV占测试样本的22%；这些样本中的其他78%用这些探针仍无法分型。据我们所知，这是美国首次报道在BRV分离株中鉴定出轮状病毒血清型G1、G2、G3和G11。
