Yuan Lijuan, Honma Shinjiro, Kim Inyoung, Kapikian Albert Z, Hoshino Yasutaka
Epidemiology Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-8026, USA.
J Infect Dis. 2009 Nov 1;200(9):1443-51. doi: 10.1086/606116.
In a study performed in 1983, 18 adult volunteers received oral challenge with the virulent human rotavirus strain D (G1P1A[8],NSP4[B]). To identify correlates of resistance to rotavirus infection, we analyzed levels of serum immunoglobulin (Ig) A and IgG antibodies to various rotaviral antigens in 16 of the 18 volunteers.
We used immunocytochemical assays that involved a total of 16 different recombinant baculoviruses, with each baculovirus expressing one of the following major serotype/genotype rotavirus proteins for the serologic assays: (1) viral protein (VP) 4 with P1A[8], P1B[4], P2A[6], P3[9], or P4[10] specificity; (2) VP7 with G1-G4 or G9 specificity; and (3) nonstructural viral protein (NSP) 4 with genotype A, B, C, or D specificity.
The prechallenge titers of IgG antibody to VP7 types G1, G3, G4, and G9; VP4 types P1A[8], P1B[4], P2A[6], and P4[10]; and NSP4 type [A] in the group of noninfected volunteers (n = 11) were significantly higher than those in the group of infected volunteers (n = 5; of these 5 volunteers, 4 were symptomatically infected). Moreover, logistic regression analysis showed that resistance to rotavirus infection most closely correlated with higher prechallenge titers of IgG antibody to homotypic VP7 (G1) and VP4 (P1A[8]).
These results suggest that protection against rotavirus infection and disease is primarily VP7/VP4 homotypic and, to a lesser degree, heterotypic.
在1983年进行的一项研究中，18名成年志愿者接受了强毒性人轮状病毒D株（G1P1A[8]，NSP4[B]）的口服激发试验。为了确定对轮状病毒感染的抗性相关因素，我们分析了18名志愿者中16人的血清免疫球蛋白（Ig）A和IgG抗体针对各种轮状病毒抗原的水平。
我们使用了免疫细胞化学分析方法，总共涉及16种不同的重组杆状病毒，每种杆状病毒表达以下主要血清型/基因型轮状病毒蛋白之一用于血清学分析：（1）具有P1A[8]、P1B[4]、P2A[6]、P3[9]或P4[10]特异性的病毒蛋白（VP）4；（2）具有G1 - G4或G9特异性的VP7；以及（3）具有A、B、C或D基因型特异性的非结构病毒蛋白（NSP）4。
未感染志愿者组（n = 11）中针对G1、G3、G4和G9型VP7；P1A[8]、P1B[4]、P2A[6]和P4[10]型VP4；以及A型NSP4的IgG抗体激发前滴度显著高于感染志愿者组（n = 5；在这5名志愿者中，4人有症状性感染）。此外，逻辑回归分析表明，对轮状病毒感染的抗性与针对同型VP7（G1）和VP4（P1A[8]）较高的IgG抗体激发前滴度最为密切相关。
这些结果表明，针对轮状病毒感染和疾病的保护主要是VP7/VP4同型的，在较小程度上是异型的。
