• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

初步鉴定 UDP-葡萄糖脱氢酶为乳腺癌患者的预后标志物，其促进表阿霉素耐药并调节 MDA-MB-231 细胞中的透明质酸合成。

Initial Identification of UDP-Glucose Dehydrogenase as a Prognostic Marker in Breast Cancer Patients, Which Facilitates Epirubicin Resistance and Regulates Hyaluronan Synthesis in MDA-MB-231 Cells.

机构信息

Laboratorio de Microambiente Tumoral, Centro de Investigaciones Básicas y Aplicadas (CIBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Junín 6000, Argentina.

Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA), UNNOBA-UNSAdA-CONICET, Junín 6000, Argentina.

出版信息

Biomolecules. 2021 Feb 9;11(2):246. doi: 10.3390/biom11020246.

DOI:10.3390/biom11020246
PMID:33572239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7914570/
Abstract

UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan-Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal microscopy. Epirubicin accumulation increased and apoptosis decreased during UGDH knockdown. Hyaluronan-coated matrix increased and a positive modulation of autophagy was detected. Higher levels of UGDH were correlated with worse prognosis in triple-negative breast cancer patients that received chemotherapy. High expression of UGDH was found in tumoral tissue from HER2-patients. However, UGDH knockdown contributes to epirubicin resistance, which might be associated with increases in the expression, deposition and catabolism of hyaluronan. The results obtained allowed us to propose UGDH as a new prognostic marker in breast cancer, positively associated with development of epirubicin resistance and modulation of extracellular matrix.

摘要

UDP-葡萄糖脱氢酶 (UGDH) 合成 UDP-葡萄糖醛酸。它参与表阿霉素解毒和透明质酸合成。本工作旨在评估 UGDH 敲低对 MDA-MB-231 乳腺癌细胞中表阿霉素反应和透明质酸代谢的影响。此外，还旨在确定 UGDH 是否可作为乳腺癌的预后标志物。我们研究了乳腺癌患者肿瘤和相邻组织中的 UGDH 表达。使用公共 Kaplan-Meier 绘图仪研究了 UGDH 的预后价值。MDA-MB-231 细胞敲低 UGDH 并接受表阿霉素治疗。通过流式细胞术分析表阿霉素蓄积和细胞凋亡。通过 Western blot 和共聚焦显微镜研究自噬 LC3-II。UGDH 敲低后，表阿霉素蓄积增加，细胞凋亡减少。透明质酸涂层基质增加，并检测到自噬的正调控。在接受化疗的三阴性乳腺癌患者中，UGDH 水平较高与预后较差相关。在 HER2 患者的肿瘤组织中发现 UGDH 高表达。然而，UGDH 敲低导致表阿霉素耐药，这可能与透明质酸的表达、沉积和分解代谢增加有关。研究结果表明，UGDH 可作为乳腺癌的新预后标志物，与表阿霉素耐药的发展和细胞外基质的调节呈正相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/8bcfedfa2106/biomolecules-11-00246-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/6cc3973b1ec7/biomolecules-11-00246-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/f99059d55b28/biomolecules-11-00246-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/e04f75a01cb8/biomolecules-11-00246-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/28c6394669d0/biomolecules-11-00246-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/b56861a80776/biomolecules-11-00246-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/7e241a978a98/biomolecules-11-00246-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/08c310abb64f/biomolecules-11-00246-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/410caff7af09/biomolecules-11-00246-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/8bcfedfa2106/biomolecules-11-00246-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/6cc3973b1ec7/biomolecules-11-00246-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/f99059d55b28/biomolecules-11-00246-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/e04f75a01cb8/biomolecules-11-00246-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/28c6394669d0/biomolecules-11-00246-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/b56861a80776/biomolecules-11-00246-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/7e241a978a98/biomolecules-11-00246-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/08c310abb64f/biomolecules-11-00246-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/410caff7af09/biomolecules-11-00246-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87ac/7914570/8bcfedfa2106/biomolecules-11-00246-g009.jpg

相似文献

1
Initial Identification of UDP-Glucose Dehydrogenase as a Prognostic Marker in Breast Cancer Patients, Which Facilitates Epirubicin Resistance and Regulates Hyaluronan Synthesis in MDA-MB-231 Cells.初步鉴定 UDP-葡萄糖脱氢酶为乳腺癌患者的预后标志物，其促进表阿霉素耐药并调节 MDA-MB-231 细胞中的透明质酸合成。
Biomolecules. 2021 Feb 9;11(2):246. doi: 10.3390/biom11020246.
2
Regulation of UDP-glucose dehydrogenase is sufficient to modulate hyaluronan production and release, control sulfated GAG synthesis, and promote chondrogenesis.UDP-葡萄糖脱氢酶的调节足以调节透明质酸的产生和释放，控制硫酸化 GAG 的合成，并促进软骨形成。
J Cell Physiol. 2011 Mar;226(3):749-61. doi: 10.1002/jcp.22393.
3
Androgen-stimulated UDP-glucose dehydrogenase expression limits prostate androgen availability without impacting hyaluronan levels.雄激素刺激的UDP-葡萄糖脱氢酶表达限制前列腺雄激素的可用性，而不影响透明质酸水平。
Cancer Res. 2009 Mar 15;69(6):2332-9. doi: 10.1158/0008-5472.CAN-08-3083. Epub 2009 Feb 24.
4
Molecular cloning and characterization of UDP-glucose dehydrogenase from the amphibian Xenopus laevis and its involvement in hyaluronan synthesis.非洲爪蟾UDP-葡萄糖脱氢酶的分子克隆、特性分析及其在透明质酸合成中的作用
J Biol Chem. 2006 Mar 24;281(12):8254-63. doi: 10.1074/jbc.M508516200. Epub 2006 Jan 17.
5
TGF-β plays a vital role in triple-negative breast cancer (TNBC) drug-resistance through regulating stemness, EMT and apoptosis.TGF-β 通过调节干性、EMT 和细胞凋亡在三阴性乳腺癌（TNBC）耐药中发挥重要作用。
Biochem Biophys Res Commun. 2018 Jul 7;502(1):160-165. doi: 10.1016/j.bbrc.2018.05.139. Epub 2018 May 24.
6
Inhibition of human UDP-glucose dehydrogenase expression using siRNA expression vector in breast cancer cells.使用小干扰RNA表达载体抑制乳腺癌细胞中人类UDP-葡萄糖脱氢酶的表达。
Biotechnol Lett. 2005 Aug;27(16):1229-32. doi: 10.1007/s10529-005-0022-z.
7
Udp-glucose dehydrogenase as a novel field-specific candidate biomarker of prostate cancer.尿苷二磷酸葡萄糖脱氢酶作为前列腺癌的一种新型的组织特异性候选生物标志物。
Int J Cancer. 2010 Jan 15;126(2):315-27. doi: 10.1002/ijc.24820.
8
Clinicopathological and prognostic significance of nuclear UGDH localization in lung adenocarcinoma.核UDP-葡萄糖脱氢酶（UGDH）定位在肺腺癌中的临床病理及预后意义
Biomed Res. 2019;40(1):17-27. doi: 10.2220/biomedres.40.17.
9
Divergent Sp1 protein levels may underlie differential expression of UDP-glucose dehydrogenase by fibroblasts: role in susceptibility to orbital Graves disease.差异表达的 Sp1 蛋白水平可能是成纤维细胞中 UDP-葡萄糖脱氢酶表达差异的基础：在眼眶格雷夫斯病易感性中的作用。
J Biol Chem. 2011 Jul 8;286(27):24487-99. doi: 10.1074/jbc.M111.241166. Epub 2011 May 16.
10
UDP-glucose dehydrogenase: structure and function of a potential drug target.UDP-葡萄糖脱氢酶：一个潜在药物靶点的结构与功能。
Biochem Soc Trans. 2010 Oct;38(5):1378-85. doi: 10.1042/BST0381378.

引用本文的文献

1
Pan-cancer analysis of UDP-glucose 6-dehydrogenase in human tumors and its function in hepatocellular carcinoma.人类肿瘤中UDP-葡萄糖6-脱氢酶的泛癌分析及其在肝细胞癌中的功能
World J Gastrointest Oncol. 2025 Jul 15;17(7):105981. doi: 10.4251/wjgo.v17.i7.105981.
2
Downregulation of UDP-glucose 6-dehydrogenase predicts adverse outcomes in patients with colorectal cancer and promotes tumorigenesis.UDP-葡萄糖6-脱氢酶的下调预示着结直肠癌患者的不良预后并促进肿瘤发生。
Sci Rep. 2025 Jul 1;15(1):21522. doi: 10.1038/s41598-025-07889-4.
3
Differential Regulation of Hyaluronan Synthesis by Three Isoforms of Hyaluronan Synthases in Mammalian Cells.

本文引用的文献

1
Cell Energy Metabolism and Hyaluronan Synthesis.细胞能量代谢与透明质酸合成。
J Histochem Cytochem. 2021 Jan;69(1):35-47. doi: 10.1369/0022155420929772. Epub 2020 Jul 6.
2
Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer. Screening of Potential Progression Markers Using qPCR.透明质酸代谢与乳腺癌和结直肠癌中的DNA修复基因相关。使用定量聚合酶链反应筛选潜在的进展标志物。
Biomedicines. 2020 Jun 29;8(7):183. doi: 10.3390/biomedicines8070183.
3
SETD3 acts as a prognostic marker in breast cancer patients and modulates the viability and invasion of breast cancer cells.
哺乳动物细胞中三种透明质酸合酶同工型对透明质酸合成的差异调节
Biomolecules. 2024 Dec 9;14(12):1567. doi: 10.3390/biom14121567.
4
Deciphering Drug Resistance: Investigating the Emerging Role of Hyaluronan Metabolism and Signaling and Tumor Extracellular Matrix in Cancer Chemotherapy.解析耐药性：研究透明质酸代谢和信号转导以及肿瘤细胞外基质在癌症化疗中的新作用。
Int J Mol Sci. 2024 Jul 11;25(14):7607. doi: 10.3390/ijms25147607.
5
Targeting hyaluronan metabolism-related molecules associated with resistant tumor-initiating cells potentiates chemotherapy efficacy in lung cancer.靶向与耐药肿瘤起始细胞相关的透明质酸代谢相关分子可增强肺癌的化疗疗效。
Sci Rep. 2024 Jul 22;14(1):16803. doi: 10.1038/s41598-024-66914-0.
6
Hypoxia-induced miR-181a-5p up-regulation reduces epirubicin sensitivity in breast cancer cells through inhibiting EPDR1/TRPC1 to activate PI3K/AKT signaling pathway.低氧诱导 miR-181a-5p 的上调通过抑制 EPDR1/TRPC1 激活 PI3K/AKT 信号通路降低乳腺癌细胞对表柔比星的敏感性。
BMC Cancer. 2024 Feb 2;24(1):167. doi: 10.1186/s12885-024-11906-6.
7
Disruption of sugar nucleotide clearance is a therapeutic vulnerability of cancer cells.糖核苷酸清除障碍是癌细胞的治疗弱点。
Nature. 2023 Nov;623(7987):625-632. doi: 10.1038/s41586-023-06676-3. Epub 2023 Oct 25.
8
UGDH promotes tumor-initiating cells and a fibroinflammatory tumor microenvironment in ovarian cancer.UGDH 促进卵巢癌中的肿瘤起始细胞和纤维炎性肿瘤微环境。
J Exp Clin Cancer Res. 2023 Oct 19;42(1):270. doi: 10.1186/s13046-023-02820-z.
9
UDP-glucose dehydrogenase (UGDH) in clinical oncology and cancer biology.尿苷二磷酸葡萄糖脱氢酶（UGDH）在临床肿瘤学和癌症生物学中的作用。
Oncotarget. 2023 Sep 28;14:843-857. doi: 10.18632/oncotarget.28514.
10
Effects of Hyaluronan on Breast Cancer Aggressiveness.透明质酸对乳腺癌侵袭性的影响。
Cancers (Basel). 2023 Jul 27;15(15):3813. doi: 10.3390/cancers15153813.
SETD3 可作为乳腺癌患者的预后标志物，并调节乳腺癌细胞的活力和侵袭性。
Sci Rep. 2020 Feb 10;10(1):2262. doi: 10.1038/s41598-020-59057-5.
4
Sirtuin 1 reduces hyaluronan synthase 2 expression by inhibiting nuclear translocation of NF-κB and expression of the long-noncoding RNA HAS2-AS1.Sirtuin 1 通过抑制 NF-κB 的核转位和长非编码 RNA HAS2-AS1 的表达来降低透明质酸合酶 2 的表达。
J Biol Chem. 2020 Mar 13;295(11):3485-3496. doi: 10.1074/jbc.RA119.011982. Epub 2020 Jan 13.
5
Emerging roles of ECM remodeling processes in cancer.ECM 重塑过程在癌症中的新作用。
Semin Cancer Biol. 2020 May;62:192-200. doi: 10.1016/j.semcancer.2019.09.004. Epub 2019 Sep 10.
6
Revisiting the hallmarks of cancer: The role of hyaluronan.重新审视癌症的特征：透明质酸的作用。
Semin Cancer Biol. 2020 May;62:9-19. doi: 10.1016/j.semcancer.2019.07.007. Epub 2019 Jul 15.
7
UDP-glucose accelerates SNAI1 mRNA decay and impairs lung cancer metastasis.UDP-葡萄糖加速 SNAI1 mRNA 衰减并损害肺癌转移。
Nature. 2019 Jul;571(7763):127-131. doi: 10.1038/s41586-019-1340-y. Epub 2019 Jun 26.
8
Proteoglycans and glycosaminoglycans as regulators of cancer stem cell function and therapeutic resistance.蛋白聚糖和糖胺聚糖作为癌症干细胞功能和治疗抵抗的调节剂。
FEBS J. 2019 Aug;286(15):2870-2882. doi: 10.1111/febs.14967. Epub 2019 Jun 27.
9
Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner.透明质酸预处理单核细胞/巨噬细胞以肿瘤类型特异性的方式影响其血管生成行为和 TSG-6 表达的调节。
FEBS J. 2019 Sep;286(17):3433-3449. doi: 10.1111/febs.14871. Epub 2019 May 16.
10
Autophagy dysfunctions associated with cancer cells and their therapeutic implications.自噬功能障碍与癌细胞及其治疗意义相关。
Biomed Pharmacother. 2019 Jul;115:108892. doi: 10.1016/j.biopha.2019.108892. Epub 2019 Apr 25.