透明质酸代谢与乳腺癌和结直肠癌中的DNA修复基因相关。使用定量聚合酶链反应筛选潜在的进展标志物。

Hyaluronan Metabolism is Associated with DNA Repair Genes in Breast and Colorectal Cancer. Screening of Potential Progression Markers Using qPCR.

作者信息

Sevic Ina, Spinelli Fiorella Mercedes, Vitale Daiana Lujan, Icardi Antonella, Romano Lucia, Brandone Alejandra, Giannoni Paula, Cristina Carolina, Bolontrade Marcela Fabiana, Alaniz Laura

机构信息

Laboratorio de Microambiente Tumoral, Centro de Investigaciones Básicas y Aplicadas (CIBA), CIT NOBA, Universidad Nacional del Noroeste de la Pcia. de Bs. As. Consejo Nacional de Investigaciones Científicas y Técnicas (UNNOBA-CONICET), Junín B6000, Argentina.

Laboratorio de Fisiopatología de la Hipófisis, Centro de Investigaciones Básicas y Aplicadas (CIBA), CIT NOBA, Universidad Nacional del Noroeste de la Pcia. de Bs. As. Consejo Nacional de Investigaciones Científicas y Técnicas (UNNOBA-CONICET), Junín B6000, Argentina.

出版信息

Biomedicines. 2020 Jun 29;8(7):183. doi: 10.3390/biomedicines8070183.

DOI:10.3390/biomedicines8070183

PMID:32610620

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7400093/

Abstract

In this work, we compared mRNA levels of Hyaluronan (HA) metabolism members and BRCA genes, known to be involved in the tumoral process, between tumor and non-tumor adjacent tissue and its correlation with previously proposed biomarkers (ER, PR, HER2 and KI67) in order to assess their value as a progression biomarkers. We show alteration in HA metabolism in colorectal but not breast cancer. However, we found a decrease in Hyaluronidase 1 HYAL1 levels in the breast but not colorectal cancer. We also show lower HA levels in tumor compared with normal tissue that could indicate a possible influence of tumor on its surrounding "normal" tissue. In both breast and colorectal cancer, CD44 and BRCA2 showed a strong positive correlation. Besides, our results show first indicators that qPCR of the analyzed genes could be used as an easy and low cost procedure for the evaluation of molecular markers we propose here.

摘要

在本研究中，我们比较了透明质酸（HA）代谢相关成员和已知参与肿瘤发生过程的BRCA基因在肿瘤组织与非肿瘤邻近组织中的mRNA水平，以及它们与先前提出的生物标志物（雌激素受体、孕激素受体、人表皮生长因子受体2和Ki-67）的相关性，以评估其作为进展生物标志物的价值。我们发现结直肠癌中HA代谢发生改变，但乳腺癌中未出现。然而，我们发现乳腺癌中透明质酸酶1（HYAL1）水平降低，而结直肠癌中未出现。我们还发现肿瘤组织中的HA水平低于正常组织，这可能表明肿瘤对其周围“正常”组织有潜在影响。在乳腺癌和结直肠癌中，CD44和BRCA2均呈强正相关。此外，我们的结果首次表明，对所分析基因进行定量聚合酶链反应可作为一种简便且低成本的方法，用于评估我们在此提出的分子标志物。