Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via A. Moro 2, 53100 Siena, Italy.
Next Technology Tecnotessile, Via del Gelso 13, 59100 Prato, Italy.
Int J Mol Sci. 2021 Feb 9;22(4):1739. doi: 10.3390/ijms22041739.
Pyomelanin mimics from homogentisic acid (HGA) and gentisic acid (GA) were biosynthesized by the oxidative enzyme laccase at physiological pH to obtain water soluble melanins. The pigments show brown-black color, broad band visible light absorption, a persistent paramagnetism and high antioxidant activity. The EPR approach shows that at least two different radical species are present in both cases, contributing to the paramagnetism of the samples. This achievement can also shed light on the composition of the ochronotic pigment in the Alkaptonuria disease. On the other hand, these soluble pyomelanin mimics, sharing physico-chemical properties with eumelanin, can represent a suitable alternative to replace the insoluble melanin pigment in biotechnological applications.
黑尿酸（HGA）和龙胆酸（GA）模拟物吡咯黑素在生理 pH 值下通过氧化酶漆酶生物合成，得到水溶性黑素。这些色素呈现出棕黑色，可见光吸收宽带，持久顺磁性和高抗氧化活性。EPR 方法表明，在这两种情况下都至少存在两种不同的自由基种类，这有助于样品的顺磁性。这一成就也可以揭示 alkaptonuria 疾病中褐素色素的组成。另一方面，这些可溶性吡咯黑素模拟物与真黑素具有相同的物理化学性质，可以作为生物技术应用中替代不溶性黑色素的合适替代品。
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