Department of Stomatology, Faculty of Medicine and Dentistry, Universitat de València, 46010 Valencia, Spain.
Department of Dermatology, Stomatology, Radiology and Physical Medicine, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain.
Dent Mater. 2021 Apr;37(4):e256-e268. doi: 10.1016/j.dental.2021.01.025. Epub 2021 Feb 8.
To present a qualitative synthesis of in vitro studies which analyzed human dental stem cell (DSC) molecular signaling pathway activation in response to hydraulic calcium silicate-based cements (HCSCs).
A systematic electronic search was performed in Medline, Scopus, Embase, Web of Science and SciELO databases on January 20 and last updated on March 20, 2020. In vitro studies assessing the implication of signaling pathways in activity related marker (gene/protein) expression and mineralization induced by HCSCs in contact with human DSCs were included.
The search identified 277 preliminary results. After discarding duplicates, and screening of titles, abstracts, and full texts, 13 articles were considered eligible. All of the materials assessed by the included studies showed positive results in cytocompatibility and/or bioactivity assays. ProRoot MTA and Biodentine were the modal HCSCs studied, hDPSCs were the modal cell variant used, and the most studied signaling pathway was MAPK. In vitro assays measuring the expression of activity-related markers and mineralized nodule formation evidenced the involvement of MAPK (and its subfamilies ERK, JNK and P38), NF-κB, Wnt/β-catenin, BMP/Smad and CAMKII pathways in the biological response of DSCs to HCSCs.
HCSCs considered in the present review elicited a favorable biological response from a variety of DSCs in vitro, thus supporting their use in biologically-based endodontic procedures. MAPK, NF-κβ, Wnt/β-catenin, BMP/Smad and CAMKII signaling pathways have been proposed as potential mediators in the biological interaction between DSCs and HCSCs. Understanding the signaling processes involved in tissue repair could lead to the development of new biomaterial compositions targeted at enhancing these mechanisms through biologically-based procedures.
对分析人牙髓干细胞（DSC）在液压钙硅基水泥（HCSCs）刺激下分子信号通路激活的体外研究进行定性综合。
于 2020 年 1 月 20 日在 Medline、Scopus、Embase、Web of Science 和 SciELO 数据库中进行系统电子检索，最后一次更新时间为 2020 年 3 月 20 日。纳入了评估 HCSCs 与人类 DSCs 接触时信号通路对活性相关标志物（基因/蛋白）表达和矿化影响的体外研究。
检索出 277 个初步结果。剔除重复项后，通过标题、摘要和全文筛选，最终有 13 项研究符合纳入标准。所有纳入研究评估的材料在细胞相容性和/或生物活性检测中均显示出阳性结果。ProRoot MTA 和 Biodentine 是研究中最常使用的 HCSCs，hDPSCs 是最常使用的细胞变体，研究最多的信号通路是 MAPK。体外检测活性相关标志物的表达和矿化结节形成的试验证明了 MAPK（及其 ERK、JNK 和 P38 亚家族）、NF-κB、Wnt/β-catenin、BMP/Smad 和 CAMKII 通路在 DSCs 对 HCSCs 生物学反应中的参与。
本综述中考虑的 HCSCs 在体外引起了多种 DSCs 的有利生物学反应，因此支持将其用于基于生物学的牙髓治疗程序。MAPK、NF-κβ、Wnt/β-catenin、BMP/Smad 和 CAMKII 信号通路已被提议作为 DSCs 和 HCSCs 之间生物学相互作用的潜在介质。了解组织修复中涉及的信号过程可能会导致开发新的生物材料组成，通过基于生物学的程序来增强这些机制。
