Department of Physiological Science, Federal University of São Carlos (UFSCar), São Carlos, Brazil.
Department of Neurosciences and Behaviour, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
Sci Rep. 2021 Feb 11;11(1):3579. doi: 10.1038/s41598-020-79168-3.
Duchene muscular dystrophy (DMD) is caused by the absence of the protein dystrophin, which leads to muscle weakness, progressive degeneration, and eventually death due to respiratory failure. Low-intensity eccentric training (LIET) has been used as a rehabilitation method in skeletal muscles after disuse. Recently, LIET has also been used for rehabilitating dystrophic muscles, but its effects are still unclear. The purpose of this study was to investigate the effects of 21 days of LIET in dystrophic soleus muscle. Thirty-six male mdx mice were randomized into six groups (n = 6/each): mdx sedentary group; mdx training group-3 days; mdx training group-21 days; wild-type sedentary group; wild-type training group-3 days and wild-type training group-21 days. After the training sessions, animals were euthanized, and fragments of soleus muscles were removed for immunofluorescence and histological analyses, and measurements of active force and Ca sensitivity of the contractile apparatus. Muscles of the mdx training group-21 days showed an improvement in morphological characteristics and an increase of active force when compared to the sedentary mdx group. The results show that LIET can improve the functionality of dystrophic soleus muscle in mice.
杜氏肌营养不良症（DMD）是由于肌营养不良蛋白的缺失引起的，导致肌肉无力、进行性退化，最终因呼吸衰竭而死亡。低强度离心训练（LIET）已被用作废用后骨骼肌的康复方法。最近，LIET 也被用于修复萎缩性肌肉，但它的效果仍不清楚。本研究的目的是研究 21 天 LIET 对萎缩性比目鱼肌的影响。36 只雄性 mdx 小鼠被随机分为六组（每组 n=6）：mdx 安静组；mdx 训练组-3 天；mdx 训练组-21 天；野生型安静组；野生型训练组-3 天和野生型训练组-21 天。训练结束后，处死动物，取出比目鱼肌片段进行免疫荧光和组织学分析，并测量收缩装置的主动力和钙敏感性。与安静的 mdx 组相比，21 天训练组的 mdx 肌肉在形态特征和主动力方面均有所改善。结果表明，LIET 可改善小鼠萎缩性比目鱼肌的功能。
