Low-intensity training provokes adaptive extracellular matrix turnover of a muscular dystrophy model.

Recommendations of therapeutic exercise in Duchenne muscular dystrophy are still controversial. The hypothesis that a low-intensity training (LIT) protocol leads to muscle adaptations on mdx mice model was tested. Dystrophic male mice with 8 weeks old were separated in exercised (mdxE, n= 8) and sedentary (mdxC, n= 8) groups. Wild-type mice were used as control (WT, n= 8) group. Exercised group underwent a LIT protocol (9 m/min, 30 min, 3 days/wk, 60 days) on a horizontal treadmill. At day 60 all animals were analyzed regarding parameters of markers of muscle lesion and extracellular matrix turnover of muscle tissue by collagens fibers on tibial anterior muscle. Histomorphometry attested that centrally located nuclei fibers and the coefficient of variance of minimal Feret's diameter was similar in mdxE and mdxC groups (= 1.000) and both groups presented higher mean values than WT group (< 0.001). Fraction area of collagen fibers of mdxE group was lower than mdxC group (= 0,027) and similar to WT group (= 0,751). Intramuscular area of Col3 of the mdxE group was higher than mdxC and WT groups (<0.001). Intramuscular area of Col1 on the mdxE group was similar to the mdxC group (= 1.000) and both groups were higher than WT group (< 0.001). LIT protocol had not influenced muscle injuries resulting from the dystrophin-deficiency membrane fragility. Although, LIT had provoked adaptations on extracellular matrix bringing higher elastic feature to dystrophic muscle tissue.