Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, UK.
Neuroscience Institute, University of Sheffield, Sheffield, UK.
Muscle Nerve. 2022 Sep;66(3):362-369. doi: 10.1002/mus.27671. Epub 2022 Jul 15.
INTRODUCTION/AIMS: Raman spectroscopy is an emerging technique for the evaluation of muscle disease. In this study we evaluate the ability of in vivo intramuscular Raman spectroscopy to detect the effects of voluntary running in the mdx model of Duchenne muscular dystrophy (DMD). We also compare mdx data with muscle spectra from human DMD patients.
Thirty 90-day-old mdx mice were randomly allocated to an exercised group (48-hour access to a running wheel) and an unexercised group (n = 15 per group). In vivo Raman spectra were collected from both gastrocnemius muscles and histopathological assessment subsequently performed. Raman data were analyzed using principal component analysis-fed linear discriminant analysis (PCA-LDA). Exercised and unexercised mdx muscle spectra were compared with human DMD samples using cosine similarity.
Exercised mice ran an average of 6.5 km over 48 hours, which induced a significant increase in muscle necrosis (P = .03). PCA-LDA scores were significantly different between the exercised and unexercised groups (P < .0001) and correlated significantly with distance run (P = .01). Raman spectra from exercised mice more closely resembled human spectra than those from unexercised mice.
Raman spectroscopy provides a readout of the biochemical alterations in muscle in both the mdx mouse and human DMD muscle.
简介/目的：拉曼光谱是一种新兴的肌肉疾病评估技术。本研究通过活体肌内拉曼光谱评估自愿跑步对 Duchenne 肌营养不良症（DMD）mdx 模型的影响。我们还将 mdx 数据与人类 DMD 患者的肌肉光谱进行了比较。
将 30 只 90 天大的 mdx 小鼠随机分为实验组（48 小时内使用跑轮）和未实验组（每组 15 只）。从两只腓肠肌中采集活体拉曼光谱，随后进行组织病理学评估。使用主成分分析-线性判别分析（PCA-LDA）对拉曼数据进行分析。使用余弦相似度将实验组和未实验组的 mdx 肌肉光谱与人类 DMD 样本进行比较。
实验组小鼠在 48 小时内平均跑步 6.5 公里，导致肌肉坏死显著增加（P=0.03）。实验组和未实验组的 PCA-LDA 评分有显著差异（P<0.0001），与跑步距离显著相关（P=0.01）。实验组小鼠的拉曼光谱比未实验组更接近人类的光谱。
拉曼光谱提供了 mdx 小鼠和人类 DMD 肌肉中肌肉生化变化的读数。
