The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.
US Department of Agriculture-Agricultural Research Service, Madison, WI, USA.
Nat Plants. 2021 Feb;7(2):198-208. doi: 10.1038/s41477-021-00854-9. Epub 2021 Feb 11.
Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ~90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologues and paralogues. We propose that Rpi-amr1 gene family diversity assists detection of diverse paralogues and alleles of the recognized effector, facilitating durable resistance against P. infestans.
晚疫病由致病疫霉引起，严重限制了马铃薯的生产。许多抗性（R）基因已从野生茄属物种中克隆，并通过杂交将其引入马铃薯品种中。然而，个别 R 基因已被致病疫霉的进化所克服；持久抗性仍然难以实现。我们从美洲马铃薯中定位克隆了一个新的 R 基因 Rpi-amr1，该基因编码一个 NRC 辅助依赖性 CC-NLR 蛋白。Rpi-amr1 在马铃薯中赋予了对所有 19 个测试的致病疫霉分离株的抗性。利用关联基因组学和长读长 RenSeq，我们从不同的美洲马铃薯和相关物种中定义了另外 8 个 Rpi-amr1 等位基因。尽管 Rpi-amr1 蛋白之间的同一性约为 90%，但所有这些等位基因都赋予了晚疫病抗性，但对 Avramr1 直系同源物和旁系同源物的识别存在差异。我们提出，Rpi-amr1 基因家族的多样性有助于检测被识别效应子的不同旁系同源物和等位基因，从而有助于对致病疫霉产生持久抗性。
