Lun Michael C, Cong Wenxiang, Arifuzzaman Md, Ranasinghe Meenakshi, Bhattacharya Sriparna, Anker Jeffery, Wang Ge, Li Changqing
Department of Bioengineering, University of California, Merced, Merced, CA 95343, USA.
Department of Biomedical Engineering, Biomedical Imaging Center, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Proc SPIE Int Soc Opt Eng. 2020 Feb;11224. doi: 10.1117/12.2544601. Epub 2020 Feb 25.
X-ray luminescence imaging emerged for about a decade and combines both the high spatial resolution of x-ray imaging with the high measurement sensitivity of optical imaging, which could result in a great molecular imaging tool for small animals. So far, there are two types of x-ray luminescence computed tomography (XLCT) imaging. One uses a pencil beam x-ray for high spatial resolution at a cost of longer measurement time. The other uses cone beam x-ray to cover the whole mouse to obtain XLCT images at a very short time but with a compromised spatial resolution. Here we review these two methods in this paper and highlight the synthesized nanophosphors by different research groups. We are building a focused x-ray luminescence tomography (FXLT) imaging system, developing a machine-learning based FXLT reconstruction algorithm, and synthesizing nanophosphors with different emission wavelengths. In this paper, we will report our current progress from these three aspects. Briefly, we mount all main components, including the focused x-ray tube, the fiber detector, and the x-ray tube and x-ray detector for a microCT system, on a rotary which is a heavy-duty ring track. A microCT scan will be performed before FXLT scan. For a FXLT scan, we will have four PMTs to measure four fiber detectors at two different wavelengths simultaneously for each linear scan position. We expect the spatial resolution of the FXLT imaging will be around 100 micrometers and a limit of detection of approximately 2 μg/mL (for GdOS:Eu).
X射线发光成像技术已经出现了大约十年，它将X射线成像的高空间分辨率与光学成像的高测量灵敏度结合在一起，有望成为一种用于小动物的强大分子成像工具。到目前为止，有两种类型的X射线发光计算机断层扫描（XLCT）成像。一种使用笔形束X射线以获得高空间分辨率，但代价是测量时间较长。另一种使用锥形束X射线覆盖整个小鼠，以在极短的时间内获得XLCT图像，但空间分辨率有所降低。在本文中，我们将对这两种方法进行综述，并重点介绍不同研究小组合成的纳米磷光体。我们正在构建一个聚焦X射线发光断层扫描（FXLT）成像系统，开发一种基于机器学习的FXLT重建算法，并合成具有不同发射波长的纳米磷光体。在本文中，我们将报告我们在这三个方面的当前进展。简而言之，我们将所有主要部件，包括聚焦X射线管、光纤探测器以及用于微型CT系统的X射线管和X射线探测器，安装在一个重型环形轨道的旋转台上。在进行FXLT扫描之前将先进行一次微型CT扫描。对于FXLT扫描，在每个线性扫描位置，我们将使用四个光电倍增管同时测量四个光纤探测器在两个不同波长下的信号。我们预计FXLT成像的空间分辨率将达到约100微米，检测限约为2μg/mL（对于GdOS:Eu）。
