补肾活血针刺抑制阿尔茨海默病SAMP8小鼠模型中NLRP1炎性小体介导的神经元焦亡

Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer's Disease.

作者信息

Zhang Ting, Guan Bin, Tan Sipin, Zhu Hong, Ren Dan, Li Ruomeng, Xiao Lan

机构信息

Department of Traditional Chinese Medicine, The Third Xiangya Hospital of Central South University, Changsha, Hunan, 410008, People's Republic of China.

Key Laboratory of Sepsis, Translational Medicine of Hunan, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, 410008, People's Republic of China.

出版信息

Neuropsychiatr Dis Treat. 2021 Feb 5;17:339-346. doi: 10.2147/NDT.S279304. eCollection 2021.

DOI:10.2147/NDT.S279304

PMID:33574670

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7872899/

Abstract

BACKGROUND

It was indicated that nucleotide-binding oligomerisation domain‑like receptor protein 1 (NLRP1) inﬂammasome-mediated pyroptosis is involveg in the progression of Alzheimer's disease (AD). This study was designed to explore the effect of Bushen Huoxue Acupuncture on cognitive defect and NLRP1 inflammasome-mediated pyroptosis in AD mouse.

METHODS

Senescence-accelerated mouse prone 8 (SAMP8) mice were used as a model of AD. Bushen Huoxue Acupuncture was performed in four acupoints: "Baihui acupoint" (GV20), "Shenshu acupoint" (BL23), "Xuehai acupoint" (SP10), and "Geshu acupoint" (BL17). Morris water maze test was performed to evaluate the cognitive function of the mouse. The levels of Aβ, Aβ, IL-1β, and IL-18 were examined by ELISA assay. Neuronal apoptosis and damage in hippocampal tissues were measured using TUNEL and Nissl staining, respectively. The expression of NLRP1, ASC, cleaved caspase-1, IL-1β, and IL-18 was examined using Western blot.

RESULTS

Bushen Huoxue Acupuncture improved the learning and memory deficits of AD mouse. Meanwhile, Bushen Huoxue Acupuncture decreased the production of Aβ in hippocampal tissues of SAMP8 mice and attenuated the neuronal apoptosis and damage. Furthermore, Bushen Huoxue Acupuncture inhibited NLRP1 inﬂammasome activation in SAMP8 mice.

CONCLUSION

Bushen Huoxue Acupuncture could notably attenuate the cognitive defect of mouse AD model and inhibit NLRP1 inflammasome-mediated pyroptosis.

摘要

背景

有研究表明，核苷酸结合寡聚化结构域样受体蛋白1（NLRP1）炎性小体介导的细胞焦亡参与了阿尔茨海默病（AD）的进展。本研究旨在探讨补肾活血针刺对AD小鼠认知缺陷及NLRP1炎性小体介导的细胞焦亡的影响。

方法

将快速老化小鼠SAMP8作为AD模型。选取“百会穴”（GV20）、“肾俞穴”（BL23）、“血海穴”（SP10）和“膈俞穴”（BL17）四个穴位进行补肾活血针刺。采用Morris水迷宫试验评估小鼠的认知功能。通过ELISA法检测Aβ、Aβ、IL-1β和IL-18的水平。分别采用TUNEL和尼氏染色法检测海马组织中的神经元凋亡和损伤情况。采用蛋白质免疫印迹法检测NLRP1、ASC、裂解的caspase-1、IL-1β和IL-18的表达。