Feng Wanguo, Zhu Ruixi, Ma Junlong, Song Han
Department of Refractive Surgery, Dalian Aier Eye Hospital, Dalian, 116092, People's Republic of China.
Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People's Republic of China.
Cancer Manag Res. 2021 Feb 5;13:1067-1073. doi: 10.2147/CMAR.S281536. eCollection 2021.
Long noncoding RNA (lncRNA) has been reported to play important roles in tumor initiation. However, how lncRNA ELFN1-AS1 affects retinoblastoma development remains unclear. Thus, we sought to elucidate its functions in retinoblastoma progression.
ELFN1-AS1 expression was measured in retinoblastoma tissues and normal tissues by qRT-PCR. CCK8, colony formation and Transwell assay were carried out to investigate the effects of ELFN1-AS1 knockdown on cell malignant behaviors. Bioinformatics analyses were performed to predict the relationship among ELFN1-AS1, miR-4270 and SBK1.
ELFN1-AS1 was highly expressed in retinoblastoma tissues and cell lines. ELFN1-AS1 was positively correlated with retinoblastoma progression and prognosis. ELFN1-AS1 knockdown curtailed retinoblastoma proliferation, migration and invasion. ELFN1-AS1 was the competing endogenous RNA for miR-4270 and promoted SBK1expression.
Altogether, our findings demonstrated that ELFN1-AS1 promotes retinoblastoma progression through mediating miR-4270/SBK1 axis and might be a promising therapeutic target.
据报道,长链非编码RNA(lncRNA)在肿瘤发生中起重要作用。然而,lncRNA ELFN1-AS1如何影响视网膜母细胞瘤的发展仍不清楚。因此,我们试图阐明其在视网膜母细胞瘤进展中的功能。
通过qRT-PCR检测视网膜母细胞瘤组织和正常组织中ELFN1-AS1的表达。进行CCK8、集落形成和Transwell实验,以研究ELFN1-AS1敲低对细胞恶性行为的影响。进行生物信息学分析,以预测ELFN1-AS1、miR-4270和SBK1之间的关系。
ELFN1-AS1在视网膜母细胞瘤组织和细胞系中高表达。ELFN1-AS1与视网膜母细胞瘤的进展和预后呈正相关。ELFN1-AS1敲低可抑制视网膜母细胞瘤的增殖、迁移和侵袭。ELFN1-AS1是miR-4270的竞争性内源性RNA,并促进SBK1的表达。
总之,我们的研究结果表明,ELFN1-AS1通过介导miR-4270/SBK1轴促进视网膜母细胞瘤的进展,可能是一个有前景的治疗靶点。