Denecker Thomas, Zhou Li Youfang, Fairhead Cécile, Budin Karine, Camadro Jean-Michel, Bolotin-Fukuhara Monique, Angoulvant Adela, Lelandais Gaëlle
Université Paris-Saclay, CEA, CNRS, Institut de Biologie Intégrative de la Cellule (I2BC), 91198, Gif-sur-Yvette, France.
Université Paris-Saclay, INRAE, CNRS, Génétique Quantitative et Évolution Le Moulon, 91400, Orsay, France.
NAR Genom Bioinform. 2020 Apr 20;2(2):lqaa027. doi: 10.1093/nargab/lqaa027. eCollection 2020 Jun.
is a cause of life-threatening invasive infections especially in elderly and immunocompromised patients. Part of human digestive and urogenital microbiota, faces varying iron availability, low during infection or high in digestive and urogenital tracts. To maintain its homeostasis, must get enough iron for essential cellular processes and resist toxic iron excess. The response of this pathogen to both depletion and lethal excess of iron at 30°C have been described in the literature using different strains and iron sources. However, adaptation to iron variations at 37°C, the human body temperature and to gentle overload, is poorly known. In this study, we performed transcriptomic experiments at 30°C and 37°C with low and high but sub-lethal ferrous concentrations. We identified iron responsive genes and clarified the potential effect of temperature on iron homeostasis. Our exploration of the datasets was facilitated by the inference of functional networks of co-expressed genes, which can be accessed through a web interface. Relying on stringent selection and independently of existing knowledge, we characterized a list of 214 genes as key elements of iron homeostasis and interesting candidates for medical applications.
是危及生命的侵袭性感染的一个病因，尤其是在老年和免疫功能低下的患者中。作为人类消化和泌尿生殖道微生物群的一部分，面临着不同的铁可用性，在感染期间较低，而在消化和泌尿生殖道中较高。为了维持其体内平衡，必须获取足够的铁用于基本的细胞过程，并抵抗过量的有毒铁。使用不同菌株和铁源，该病原体对30°C时铁缺乏和致死性铁过量的反应已在文献中有所描述。然而，对于在37°C（人体温度）下对铁变化的适应以及对轻度过载的适应，人们了解甚少。在本研究中，我们在30°C和37°C下，使用低和高但亚致死浓度的亚铁进行了转录组实验。我们鉴定了铁反应基因，并阐明了温度对铁稳态的潜在影响。通过推断共表达基因的功能网络促进了我们对数据集的探索，该网络可通过网络界面访问。依靠严格的筛选且独立于现有知识，我们将214个基因的列表表征为铁稳态的关键要素以及医学应用的有趣候选基因。
