Fly Laboratory # 210, Anusandhan Kendra-II, School of Chemical & Biotechnology, SASTRA Deemed to be University, Thanjavur, Tamil Nadu, 613401, India.
Biogerontology. 2021 Apr;22(2):253-259. doi: 10.1007/s10522-021-09912-3. Epub 2021 Feb 11.
Diet restriction (DR) studies undergo the implementation of reduced single or multiple component/s of the fly food without causing malnutrition. The question of how and why DR modifies the fate of lifespan in fruit flies Drosophila melanogaster has prompted us to emphasize by attending the control food composition first. Certain concentrations of DR food do not always confer an extended lifespan, rather it enables the flies to achieve their normal lifespan, which was probably reduced by the control food per se (having toxic effect caused due to the excess levels of dietary components). However, the current paradigm of DR studies has elicited its benefits and losses via trade-offs in the organismal traits and have highlighted the need for a common diet, but have not claimed the tested diets as balanced. So, the DR effect on lifespan and other fitness traits cannot be justified only based on varying control food across labs and hence, the approach of DR studies has to be revisited and a balanced diet has to be formulated. The current article discusses the need for a balanced diet, the traits to be considered before designing a diet, and certain problems in the existing synthetic medium. Therefore, based on the control food composition, the validity of lifespan extension conferred by these nutrient restricted diets need to be accounted for.
饮食限制（DR）研究是在不造成营养不良的情况下，减少果蝇食物中的单一或多种成分来进行的。DR 如何以及为何改变黑腹果蝇寿命命运的问题促使我们首先强调关注对照食物的组成。某些浓度的 DR 食物并不总是延长寿命，而是使果蝇能够达到其正常寿命，而正常寿命可能因对照食物本身（由于饮食成分过量而产生毒性作用）而降低。然而，目前的 DR 研究范式通过在生物体特征上的权衡利弊来体现其益处和损失，并强调了需要一种通用饮食，但并没有声称所测试的饮食是平衡的。因此，不能仅仅根据不同实验室的对照食物来证明 DR 对寿命和其他适应性特征的影响，因此必须重新审视 DR 研究方法，并制定一种平衡饮食。本文讨论了对平衡饮食的需求，在设计饮食之前需要考虑的特征，以及现有合成培养基中的某些问题。因此，基于对照食物的组成，需要考虑这些营养限制饮食赋予的延长寿命的有效性。
