Fly Laboratory # 210, Anusandhan Kendra-II, School of Chemical & Biotechnology, SASTRA Deemed to be University, Thanjavur, 613401, Tamil Nadu, India.
Sci Rep. 2020 Jun 22;10(1):10019. doi: 10.1038/s41598-020-66372-4.
Drosophila melanogaster has been used in Diet Restriction (DR) studies for a few decades now, due to easy diet implementation and its short lifespan. Since the concentration of protein determines the trade-offs between lifespan and fecundity, it is important to understand the level of protein and the extent of its influence on lifespan, fecundity and activity of fruit flies. In this study, we intend to assess the effect of a series of protein restricted diets from age 1 day of the adult fly on these traits to understand the possible variations in trade-off across tested diets. We found that lifespan under different protein concentrations remains unaltered, even though protein restricted diets exerted an age-specific influence on fecundity. Interestingly, there was no difference in lifetime activity of the flies in most of the tested protein restricted (PR) diets, even though a sex-dependent influence of protein concentrations was observed. Additionally, we report that not all concentrations of PR diet increase activity, thereby suggesting that the correlation between lifespan and the lifetime activity can be challenged under protein-restricted condition. Therefore, the PR does not need to exert its effect on lifespan and fecundity only but can also influence activity levels of the flies, thereby emphasizing the role of nutrient allotment between lifespan, fecundity and activity.
黑腹果蝇由于其易于进行饮食控制和较短的寿命，已被用于饮食限制（DR）研究几十年。由于蛋白质浓度决定了寿命和繁殖力之间的权衡取舍，因此了解蛋白质的水平及其对寿命、繁殖力和果蝇活力的影响程度非常重要。在这项研究中，我们旨在评估一系列成年果蝇从 1 日龄开始的蛋白质限制饮食对这些特征的影响，以了解在测试的饮食中可能存在的权衡变化。我们发现，不同蛋白质浓度下的寿命保持不变，尽管蛋白质限制饮食对繁殖力有特定的年龄影响。有趣的是，在大多数测试的蛋白质限制（PR）饮食中，果蝇的终生活动没有差异，尽管观察到了蛋白质浓度的性别依赖性影响。此外，我们报告称并非所有 PR 饮食浓度都能增加活动，这表明在蛋白质限制条件下，寿命和终生活动之间的相关性可能受到挑战。因此，PR 不仅需要对寿命和繁殖力产生影响，还可以影响果蝇的活动水平，从而强调了寿命、繁殖力和活动之间营养分配的作用。
