Fuchino Hiroyuki, Murase Sayaka, Hishida Atsuyuki, Kawahara Nobuo
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 1-2 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan.
Hokkaido Division, Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 108-4, Ohashi, Nayoro, Hokkaido, 096-0065, Japan.
J Nat Med. 2021 Jun;75(3):499-519. doi: 10.1007/s11418-021-01486-1. Epub 2021 Feb 11.
Differences in the components of the crude drug Saposhnikoviae radix, both wild and cultivated, and the cultivation duration were examined by UHPLC/MS. As a result, there was no significant difference in composition depending on the region in China where the drug was produced. The most abundant components in all samples were prim-O-glucosylcimifugin, 4'-O-glucosyl-5-O-methylvisamminol, 3'-O-acetylhamaudol and cimifugin. The 1 year-old Saposhnikoviae radix cultivated in Japan had a low component content overall. A comparison of components according to root thickness revealed that glycosides, such as prim-O-glucosylcimifugin and 4'-O-glucosyl-5-O-methylvisamminol, were accumulated in thin roots. In a comparison of the components according to the drying temperature, a large difference was observed in the content of glycosides, and a difference was observed depending on the sugar-binding position. According to a metabolome analysis in domestic commercial products by LC/MS, a characteristic component in the cultivated product was found and its content was low in the 1 year-old sample and high in the 2 year-old sample. If the cultivation duration is prolonged up to about 6 years, the contents of the ingredients are close to those of wild products.
采用超高效液相色谱/质谱联用技术（UHPLC/MS）对野生和栽培防风药材的成分差异以及栽培年限进行了研究。结果表明，药材产地在中国不同地区，其成分无显著差异。所有样品中含量最高的成分是升麻素苷、5-O-甲基维斯阿米醇苷、3'-O-乙酰哈巴俄苷和升麻素。在日本栽培的1年生防风药材总体成分含量较低。根据根的粗细对成分进行比较发现，升麻素苷和5-O-甲基维斯阿米醇苷等糖苷类成分在细根中积累。根据干燥温度对成分进行比较，发现糖苷类成分的含量有很大差异，且根据糖结合位置也存在差异。通过液相色谱/质谱联用技术（LC/MS）对国内商业产品进行代谢组学分析，发现了栽培产品中的一种特征成分，其在1年生样品中的含量较低，在2年生样品中的含量较高。如果将栽培年限延长至约6年，其成分含量接近野生产品。
