Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Medical College, Harbin Vocational & Technical College, Harbin, Heilongjiang, China.
PeerJ. 2022 Nov 4;10:e14336. doi: 10.7717/peerj.14336. eCollection 2022.
is a traditional Chinese herb that mainly grows in arid grasslands and strongly adapts to various stresses. Drought is not only a major abiotic stress factor but also a typical feature conducive to producing high-quality medicinal material. The present study investigated by treating plants with polyethylene glycol (PEG-6000). Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) identified 146 compounds from the roots of , among which seven primary metabolites and 28 secondary metabolites showed significant changes after drought treatment. UV-Vis spectrophotometer detected the activity of antioxidant enzymes and the content of superoxide anion (O ) and (MDA). The differential primary metabolites revealed that drought promotes glycolysis, reducing primary metabolism and enhancing secondary metabolism. Meanwhile, the differential secondary metabolites showed an increase in the content of compounds upstream of the secondary metabolic pathway, and other glycosides and increased that of the corresponding aglycones. The activities of antioxidant enzymes and the content of O and MDA shown different changes duing the drought treatment. These observations indicate that drought promotes the biosynthesis and transformation of the secondary metabolites and activity of antioxidant enzymes, improving plant adaptability. The present study also analyzed a few primary and secondary metabolites of under different degrees and durations of drought and speculated on the metabolic pathways in an arid environment. The findings indicate the biological nature, diversity, and complexity of secondary metabolites and the mechanisms of plant adaptation to ecological stress.
是一种传统的中国草药，主要生长在干旱的草原上，能够很好地适应各种压力。干旱不仅是一种主要的非生物胁迫因素，也是产生高质量药材的典型特征。本研究通过用聚乙二醇（PEG-6000）处理 植物来进行研究。超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-TOF-MS）从 的根部中鉴定出 146 种化合物，其中七种主要代谢物和 28 种次生代谢物在干旱处理后表现出明显变化。紫外可见分光光度计检测抗氧化酶的活性以及超氧阴离子（O ）和丙二醛（MDA）的含量。差异的初级代谢物表明干旱促进了糖酵解，降低了初级代谢物并增强了次级代谢物。同时，差异的次生代谢物显示出次生代谢途径上游化合物的含量增加，以及其他糖苷的含量增加和相应的苷元的含量增加。抗氧化酶的活性和 O 以及 MDA 的含量在干旱处理过程中表现出不同的变化。这些观察结果表明，干旱促进了次生代谢物和抗氧化酶的生物合成和转化，提高了植物的适应性。本研究还分析了 在不同程度和持续时间的干旱下的一些初级和次级代谢物，并推测了在干旱环境中的代谢途径。研究结果表明了次生代谢物的生物特性、多样性和复杂性，以及植物适应生态胁迫的机制。
