通过 EGFR 抑制对柔毛淫羊藿选择性细胞毒性极性部位的化学成分分析及其在 HepG2 细胞中的作用

Chemical Constituent Profiling of var. Pubescens with Selective Cytotoxic Polar Fraction through EGFR Inhibition in HepG2 Cells.

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.

Garden for Medicinal Plants, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-Machi 1-14, Nagasaki 852-8521, Japan.

出版信息

Molecules. 2021 Feb 10;26(4):940. doi: 10.3390/molecules26040940.

Different extracts of the Bamboo shoot skin var. pubescens were screened against panel of cancer cell lines and normal one. The cell viability results exhibited that the ethyl acetate extract showed the least vitality percentage of 2.14% of HepG2 cells. Accordingly, it was subjected to chromatographic separation, which resulted in the isolation of a new natural product; 7-hydroxy, 5-methoxy, methyl cinnamate (), together with four known compounds. The structures of the pure isolated compounds were deduced based on different spectroscopic data. The new compound () was screened against the HepG2 and MCF-7 cells and showed IC values of 7.43 and 10.65 µM, respectively. It induced apoptotic cell death in HepG2 with total apoptotic cell death of 58.6% (12.44-fold) compared to 4.71% in control by arresting cell cycle progression at the G1 phase. Finally, compound was validated as EGFR tyrosine kinase inhibitor in both enzymatic levels (IC = 98.65 nM compared to Erlotinib (IC = 78.65 nM). Finally, in silico studies of compound through the molecular docking indicated its high binding affinity towards EGFR protein and the ADME pharmacokinetics indicated it as a drug-like.

不同提取部位的毛竹皮 var. pubescens 进行了针对肿瘤细胞系和正常细胞的筛选。细胞活力结果表明，乙酸乙酯提取物对 HepG2 细胞的活力百分比最低，为 2.14%。因此，对其进行了色谱分离，得到了一个新的天然产物；7-羟基、5-甲氧基、肉桂酸甲酯（），以及四个已知化合物。根据不同的光谱数据推导出了纯分离化合物的结构。新化合物（）对 HepG2 和 MCF-7 细胞进行了筛选，其 IC 值分别为 7.43 和 10.65 µM。与对照组相比，它通过将细胞周期阻滞在 G1 期，诱导 HepG2 细胞发生总凋亡细胞死亡，凋亡细胞死亡达到 58.6%（12.44 倍）。最后，化合物被验证为 EGFR 酪氨酸激酶抑制剂，在酶水平上的 IC 为 98.65 nM，与厄洛替尼（IC = 78.65 nM）相比。最后，通过分子对接对化合物进行了计算机模拟研究，表明其对 EGFR 蛋白具有高结合亲和力，ADME 药代动力学表明其具有类药性。