Chitiva Luis Carlos, Rezende-Teixeira Paula, Leão Tiago F, Lozano-Puentes Hair Santiago, Londoño Ximena, Díaz-Ariza Lucía Ana, Costa-Lotufo Leticia V, Prieto-Rodríguez Juliet A, Costa Geison M, Castro-Gamboa Ian
Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais (NuBBE), Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo 14800-901, Brazil.
Grupo de Investigación Fitoquímica Universidad Javeriana (GIFUJ), Department of Chemistry, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia.
ACS Omega. 2024 Aug 21;9(35):36939-36960. doi: 10.1021/acsomega.3c09114. eCollection 2024 Sep 3.
Bamboo plants are widely used in Asian traditional medicine for various health issues and exhibit therapeutic potential. species are renowned bamboos for their high phenolic compound content, including flavonoids and hydroxycinnamic acid derivatives, and possess noteworthy biological properties. Despite this, there is a notable scarcity of research on the chemical and biological aspects of Latin American bamboo leaf extracts (BLEs), especially concerning the genus. This study aimed to employ a metabolomics approach to integrate the phytochemical and activity profiles of BLEs to identify potential bioactive markers. We determined the metabolic fingerprints of 30 BLEs through HPTLC, HPLC-DAD, UHPLC-QTOF-MS, and H-NMR analyses and screened for antioxidant and cytotoxic activities using ABTS, DPPH, and MTT methods. Ultimately, correlation analyses were performed by using chemometric methods and molecular networking. Our findings present a comprehensive chemical characterization, encompassing 40 flavonoids and 9 cinnamic acid derivatives. Notably, most of these compounds have been reported for the first time within the genus, signifying novel discoveries. Additionally, certain compounds identified in other species of the subfamily Bambusoideae provide valuable comparative insights. These compounds demonstrated a significant correlation with antioxidant potential, with values exceeding 100 and 30 μmol of TE/g of extract for ABTS and DPPH, respectively, in the samples. Extracts from and exhibited potent cytotoxic effects with IC values of 1.23 and 4.73 μg/mL against HCT-116 colon cancer cells, respectively. Notably, glycosylated flavones showed a strong correlation with cytotoxicity. These new findings significantly contribute to our understanding of the chemical composition and biological properties of these often overlooked bamboo species, providing them with important added value and alternative use.
竹子在亚洲传统医学中被广泛用于治疗各种健康问题，并具有治疗潜力。某些竹种因其高酚类化合物含量（包括黄酮类化合物和羟基肉桂酸衍生物）而闻名，并具有显著的生物学特性。尽管如此，关于拉丁美洲竹叶提取物（BLEs）的化学和生物学方面的研究明显匮乏，尤其是关于[具体竹属]的研究。本研究旨在采用代谢组学方法整合BLEs的植物化学和活性谱，以确定潜在的生物活性标记物。我们通过高效薄层色谱（HPTLC）、高效液相色谱-二极管阵列检测（HPLC-DAD）、超高效液相色谱-四极杆飞行时间质谱（UHPLC-QTOF-MS）和氢核磁共振（H-NMR）分析确定了30种BLEs的代谢指纹图谱，并使用ABTS、DPPH和MTT方法筛选了抗氧化和细胞毒性活性。最终，通过化学计量学方法和分子网络进行了相关性分析。我们的研究结果呈现了全面的化学表征，包括40种黄酮类化合物和9种肉桂酸衍生物。值得注意的是，这些化合物中的大多数是首次在该竹属中被报道，这意味着有新的发现。此外，在竹亚科其他物种中鉴定出的某些化合物提供了有价值的比较见解。这些化合物与抗氧化潜力显示出显著相关性，在样品中，ABTS和DPPH的抗氧化能力分别超过100和30 μmol TE/g提取物。来自[具体竹种1]和[具体竹种2]的提取物对HCT-116结肠癌细胞表现出较强的细胞毒性作用，IC50值分别为1.23和4.73 μg/mL。值得注意的是，糖基化黄酮与细胞毒性显示出很强的相关性。这些新发现显著有助于我们理解这些常被忽视的竹种的化学成分和生物学特性，为它们提供了重要的附加价值和替代用途。
