播散性微孢子虫病的诊断：检测HIV/AIDS患者血液中循环的比氏肠微孢子虫DNA。

Diagnosis of disseminated microsporidiosis: Detection of circulating Enterocytozoon bieneusi DNA in blood of HIV/AIDS patients.

作者信息

Zainudin N S, Nasarudin S N S, Periyasamy P, Moktar N, Noordin R, Osman E

机构信息

Department of Parasitology and Medical Entomology, Medical Faculty, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaakob Latiff, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.

Department of Medicine, Medical Faculty, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaakob Latiff, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.

出版信息

Trop Biomed. 2016 Dec 1;33(4):761-770.

PMID:33579073

Abstract

Disseminated microsporidiosis among HIV/AIDS patients is life-threatening. The incidence may be actually higher than what has been reported. This is due to non-specific presentations of the disseminated cases and also the insensitivity of routine diagnostic technique which contribute to delay in the treatment of the disease. In the present study, we report the use of blood specimens to detect circulating microsporidia DNA, which has not been reported for diagnosis of disseminated microsporidiosis. Blood samples from HIV/AIDSpositive patients were collected over a period of one year. These samples were subjected to PCR assay using species-specific primer EBIEF1/EBIER1. Out of 100 patients, seven were confirmed positive for E. bieneusi by PCR. A fragment of 607 bp was successfully amplified. Identification of circulating microsporidia DNA in blood samples may aid in early diagnosis, thereby allows timely administration of anti-parasitic treatment.

摘要

HIV/AIDS患者中的播散性微孢子虫病会危及生命。其发病率可能实际上高于已报道的情况。这是由于播散性病例的表现不具特异性，以及常规诊断技术的不敏感性，这些因素导致了疾病治疗的延迟。在本研究中，我们报告了使用血液标本检测循环微孢子虫DNA的情况，此前尚未有关于此用于诊断播散性微孢子虫病的报道。在一年时间里收集了HIV/AIDS阳性患者的血液样本。使用种特异性引物EBIEF1/EBIER1对这些样本进行PCR检测。在100名患者中，有7名通过PCR被确诊为贝氏等孢球虫阳性。成功扩增出了一段607 bp的片段。鉴定血液样本中的循环微孢子虫DNA可能有助于早期诊断，从而能够及时给予抗寄生虫治疗。