Cirkovic V, Stamenkovic G, Jovanovic J, Siljic M, Paunovic M, Stanojevic M
University of Belgrade, School of Medicine, Belgrade, Serbia.
University of Belgrade, Institute for Biological Research "Siniša Stankovic", Belgrade, Serbia.
Trop Biomed. 2016 Dec 1;33(4):780-785.
Bats represent a known reservoir of emerging viruses, yet no molecular data are found about the occurrence of zoonotic viruses in bats in the Balkans. The aim of this study was to determine the presence of paramyxo- and hanta-viruses in bats, examined by PCR in 95 deceased bats, that were collected in Serbia and Montenegro, during the period 2002 to 2009. All samples tested positive for beta-actin mRNA, confirming successful RNA isolation and amplification. However, no sample tested positive for virus specific RNA. Our findings might reflect tissue degradation in carcass samples and do not exclude bats as potential viral reservoir in the surveyed geographic area.
蝙蝠是已知的新兴病毒宿主,但巴尔干地区蝙蝠中是否存在人畜共患病毒尚无分子数据。本研究的目的是通过PCR检测2002年至2009年期间在塞尔维亚和黑山收集的95只死亡蝙蝠,以确定其中是否存在副粘病毒和汉坦病毒。所有样本的β-肌动蛋白mRNA检测均呈阳性,证实RNA成功分离和扩增。然而,没有样本的病毒特异性RNA检测呈阳性。我们的研究结果可能反映了尸体样本中的组织降解情况,并且不能排除蝙蝠在被调查地理区域作为潜在病毒宿主的可能性。