Department of Ophthalmology, University of Minnesota, Minneapolis, MN, USA.
Department of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.
Stem Cell Res Ther. 2021 Feb 12;12(1):122. doi: 10.1186/s13287-021-02184-1.
This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.
这项研究首次使用胚胎互补法在 Neurog1 杂合子小鼠中产生诱导多能干细胞源性内耳感觉神经元。此外，该方法纠正了与 Neurog1 杂合性相关的非感觉缺陷，表明互补作用是针对内源性 Neurog1 功能的。这项工作验证了胚胎互补法作为一种工具的使用，可深入了解发育基因的功能，并强调胚胎互补法作为一种潜在的平台，可用于产生可移植到受损内耳以改善听力的嵌合内耳细胞类型。
