Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araraquara, Brazil.
Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, Brazil.
Dent Mater. 2021 Jun;37(6):e329-e340. doi: 10.1016/j.dental.2021.01.014. Epub 2021 Feb 10.
To investigate the trans-enamel and trans-dentinal biological effects of treating enamel white spot-like lesions (EWSLs) with resin infiltration components (RICs) on odontoblast-like cells (MDPC-23) and human dental pulp cells (HDPCs).
EWSLs were induced in 60 enamel/dentin discs (4.0 ± 0.2 mm thick) using S. mutans. The discs were adapted into artificial pulp chambers and MDPC-23 were seeded on the dentin surface. The components of a resin infiltration system (Icon) were applied individually or in combination on the enamel surface as following (n = 10/treatment): Etch, Infiltrant, Etch+Infiltrant, or Etch+Dry+Infiltrant. The application of water or hydrogen peroxide served as negative and positive controls, respectively. After 72 h, MDPC-23 viability was evaluated. The extracts were exposed for 72 h to pre-cultured MDPC-23 and HDPCs in 96-well plates to evaluate cell viability, alkaline phosphatase activity (ALP), mineralized nodule formation (MN), and the expression of inflammatory cytokines (ICs) and mineralization-related genes (MRs). Data were analyzed by ANOVA complemented with Tukey or Games-Howell post-hocs (α = 5%).
Cell viability, ALP activity, and MN formation were significantly reduced in response to the RICs, presenting intermediate values compared to positive and negative controls. Likewise, ICs were upregulated, whereas MRs were downregulated. Among the RICs, the Etch component caused the most notorious detrimental effects.
Resin infiltration of EWSLs negatively affected the metabolism of pulp cells in vitro. Therefore, even though resin infiltration is a micro-invasive therapy for non-cavitated caries in enamel, it should be closely followed up seen that components may diffuse and unbalance pulp homeostasis.
研究使用树脂渗透成分（RIC）治疗釉质白斑样病变（EWSL）对成牙本质样细胞（MDPC-23）和人牙髓细胞（HDPCs）的牙本质内和牙本质外的生物学影响。
使用 S. mutans 在 60 个釉质/牙本质圆盘（4.0 ± 0.2 mm 厚）上诱导 EWSL。将圆盘适应到人工牙髓腔中，并在牙本质表面接种 MDPC-23。单独或联合将树脂渗透系统（Icon）的成分应用于釉质表面，如下所示（n = 10/处理）：蚀刻、渗透、蚀刻+渗透、或蚀刻+干燥+渗透。水或过氧化氢的应用分别作为阴性和阳性对照。72 h 后，评估 MDPC-23 活力。将提取物暴露于预培养的 MDPC-23 和 HDPCs 中 72 h，在 96 孔板中评估细胞活力、碱性磷酸酶活性（ALP）、矿化结节形成（MN）以及炎症细胞因子（ICs）和矿化相关基因（MRs）的表达。使用方差分析（ANOVA），并用 Tukey 或 Games-Howell 事后检验（α = 5%）进行补充分析。
RIC 处理后细胞活力、ALP 活性和 MN 形成显著降低，与阳性和阴性对照相比呈现中间值。同样，ICs 上调，而 MRs 下调。在 RIC 中，蚀刻成分引起的有害影响最为明显。
EWSL 的树脂渗透对体外牙髓细胞的代谢产生负面影响。因此，尽管树脂渗透是釉质非龋性龋的微创治疗方法，但应密切随访，因为成分可能扩散并破坏牙髓的内稳态平衡。
