Stem Cell Technology Research Center, Tehran, Iran.
Science and Research Branch, Islamic Azad University, Tehran, Iran.
In Vitro Cell Dev Biol Anim. 2021 Mar;57(3):290-299. doi: 10.1007/s11626-020-00430-z. Epub 2021 Feb 12.
Application of hyperosmolarity can be a promising strategy to promote chondrogenic differentiation in adipose-derived mesenchymal stem cells (ADSCs). Growth factors may promote different signaling pathways in parallel that is why in this study we monitor undesired pathologic or unwanted side effects as well as chondroinductive impacts of hyperosmolarity in differentiating ADSCs. Quantified gene expression, immunocytochemistry, glycosaminoglycan deposition and angiogenic secretion assays performed along with immunoassay. We observed that hyperosmolarity pressure of 480 mOsm over-expressed cartilage specific markers at gene expression level in the extra cellular matrix. Meanwhile, hyperosmolarity of 480 mOsm diminished the expression of cartilage associated pathologic markers, i.e., inflammatory and angiogenic attributes. Certain dose of hyperosmolarity could benefit chondrogenesis in a dual way, first by increasing chondrogenic markers and second by lowering tissue mineralization and angiogenic potential. The chondroprotective potential of hyperosmolarity could have a promising benefit in cartilage cell therapy and tissue engineering.
高渗环境的应用可能是促进脂肪间充质干细胞（ADSCs）软骨分化的一种有前途的策略。生长因子可能会平行促进不同的信号通路，这就是为什么在这项研究中，我们监测高渗环境在诱导 ADSC 分化过程中对未期望的病理或不良的副作用以及软骨诱导的影响。通过免疫测定法同时进行定量基因表达、免疫细胞化学、糖胺聚糖沉积和血管生成分泌测定。我们观察到，480毫渗的高渗透压在细胞外基质中在基因表达水平上过度表达了软骨特异性标记物。同时，480 毫渗的高渗透压降低了与软骨相关的病理标记物的表达，即炎症和血管生成属性。一定剂量的高渗性可以双重促进软骨生成，一方面增加软骨生成标记物，另一方面降低组织矿化和血管生成潜力。高渗性的软骨保护潜力可能在软骨细胞治疗和组织工程中有很好的应用前景。
