Widyowati Retno, Suciati Suciati, Haryadi Dewi Melani, Chang Hsin-I, Suryawan Ipg Ngurah, Tarigan Nurliana
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Nanizar Zaman Joenoes Building, Campus C, Mulyorejo, Surabaya, Indonesia.
Department of Pharmaceutics, Faculty of Pharmacy, Universitas Airlangga, Nanizar Zaman Joenoes Building, Campus C, Mulyorejo, Surabaya, Indonesia.
J Basic Clin Physiol Pharmacol. 2021 Feb 15;32(6):1145-1150. doi: 10.1515/jbcpp-2020-0140.
Glucocorticoid-induced osteoporosis (dexamethasone) is a primary cause of secondary osteoporosis by the decreasing formation and increasing resorption activities. Previously, the study showed that 70% ethanol and aqueous extract of deer antler have increased alkaline phosphatase in osteoblast cell that known as marker of bone formation. The mind of this study is to analyze the effect of deer antlers in increasing the bone trabecular density of osteoporosis-induced male mice.
This study used a post-test control group design. A total of 54 healthy male mice were randomly divided to nine groups, i.e., healthy control, osteoporotic, positive control, 70% ethanol (4, 8, and 12 mg/kg BW), and aqueous extracts (4, 8, and 12 mg/kg BW) of deer antler groups. All of the interventions were given 1 mL of test sample for 4 weeks orally. The bone densities were determined using histomorphometry by Image J and Adobe Photoshop. The statistical data were performed using SPSS 23 and statistical significance was set at p<0.05.
The results showed that alendronate group, 70% ethanol, and aqueous extract groups increased bone density and calcium levels in serum (p<0.05) compared to osteoporotic group in dose dependent manner. It indicated that 70% ethanol and aqueous extract of deer antler stimulating bone turnover and aqueous extract showed the highest.
Dexamethasone induction for 4 weeks caused osteoporotic mice and the administration of 70% ethanol and aqueous extracts of deer antler from East Kalimantan increased trabecular bone density and calcium levels in dose dependent manner.
糖皮质激素诱导的骨质疏松症（地塞米松）是继发性骨质疏松症的主要原因，其通过减少骨形成和增加骨吸收活动导致该病。此前，研究表明，70%乙醇和鹿茸水提取物可增加成骨细胞中的碱性磷酸酶，而碱性磷酸酶是骨形成的标志物。本研究旨在分析鹿茸对增加骨质疏松症诱导的雄性小鼠骨小梁密度的影响。
本研究采用试验后对照组设计。总共54只健康雄性小鼠被随机分为9组，即健康对照组、骨质疏松组、阳性对照组、70%乙醇（4、8和12毫克/千克体重）组以及鹿茸水提取物（4、8和12毫克/千克体重）组。所有干预措施均口服给予1毫升测试样品，持续4周。使用Image J和Adobe Photoshop通过组织形态计量学测定骨密度。使用SPSS 23进行统计数据处理，统计学显著性设定为p<0.05。
结果显示，与骨质疏松组相比，阿仑膦酸钠组、70%乙醇组和水提取物组以剂量依赖方式增加了血清骨密度和钙水平（p<0.05）。这表明70%乙醇和鹿茸水提取物刺激了骨转换，且水提取物的效果最为显著。
地塞米松诱导4周导致小鼠患骨质疏松症，给予来自东加里曼丹的70%乙醇和鹿茸水提取物可剂量依赖性地增加骨小梁密度和钙水平。
