Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China; School of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
School of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Phytochemistry. 2021 May;185:112696. doi: 10.1016/j.phytochem.2021.112696. Epub 2021 Feb 10.
Metabolic networks can provide insight into the biosynthesis pathways of natural products present in plant-derived medicines. Here, we primarily established a highly efficient and targeted method for the systematic screening of isoquinoline alkaloids from the Macleaya genus. A total of 392 potential alkaloids were detected, 204 of which were further identified according to their tandem mass spectrometry (MS/MS) spectra and the characteristic fragmentation patterns of references. A metabolic network of isoquinoline alkaloids from the Macleaya genus was then constructed based on the structural relationships, metabolic level differences, and the isotopically labeled [ring-C]-tyrosine feeding experiments. New biosynthesis pathways for well-known alkaloids (berberine, sanguinarine, and chelerythrine) in the Macleaya genus were proposed on the basis of the established metabolic network. This work marks the first comprehensive study of the metabolic network of isoquinoline alkaloids in the Macleaya genus and provides a template for constructing the metabolic networks of other plant-derived medicines.
代谢网络可以深入了解植物药中天然产物的生物合成途径。在这里，我们主要建立了一种从博落回属植物中系统筛选异喹啉生物碱的高效靶向方法。共检测到 392 种潜在的生物碱，其中 204 种根据串联质谱（MS/MS）图谱和参考文献的特征碎片模式进一步鉴定。然后根据结构关系、代谢水平差异和同位素标记 [环-C]-酪氨酸喂养实验，构建了博落回属异喹啉生物碱的代谢网络。在此代谢网络的基础上，提出了博落回属中一些熟知生物碱（小檗碱、血根碱和白屈菜红碱）的新生物合成途径。本研究首次全面研究了博落回属异喹啉生物碱的代谢网络，为构建其他植物药的代谢网络提供了模板。
