College of Agronomy, Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Crop Growth Regulation of Hebei Province, Baoding, China.
Human Resource Department, Hebei Agricultural University, Baoding, China.
Plant Cell Environ. 2021 Jun;44(6):1769-1787. doi: 10.1111/pce.14031. Epub 2021 Mar 5.
Small heat shock proteins (sHSPs), a family of the ubiquitous stress proteins in plants acting as molecular chaperones to protect other proteins from stress-induced damage, have been implicated in plant growth and development as well as plant response to environmental stress, especially heat stress. In this study, a chloroplast-localized sHSP, AsHSP26.8, was overexpressed in creeping bentgrass (Agrostis stolonifera L.) to study its role in regulating plant growth and stress response. Transgenic (TG) creeping bentgrass plants displayed arrested root development, slow growth rate, twisted leaf blades and are more susceptible to heat and salt but less sensitive to drought stress compared to wild-type (WT) controls. RNA-seq analysis revealed that AsHSP26.8 modulated the expression of genes in auxin signalling and stress-related genes such as those encoding HSPs, heat shock factors and other transcription factors. Our results provide new evidence demonstrating that AsHSP26.8 negatively regulates plant growth and development and plays differential roles in plant response to a plethora of diverse abiotic stresses.
小分子热激蛋白（sHSPs）是植物中普遍存在的应激蛋白家族，作为分子伴侣保护其他蛋白质免受应激诱导的损伤，它们与植物的生长发育以及植物对环境应激，特别是热应激的反应有关。在这项研究中，过表达了一种定位于叶绿体的 sHSP，即 AsHSP26.8，以研究其在调节植物生长和应激反应中的作用。与野生型（WT）对照相比，转基因（TG）匍匐翦股颖植物表现出根系发育受阻、生长缓慢、叶片扭曲，对热和盐更敏感，但对干旱胁迫的敏感性较低。RNA-seq 分析显示，AsHSP26.8 调节了生长素信号和应激相关基因的表达，如 HSPs、热休克因子和其他转录因子的编码基因。我们的结果提供了新的证据，证明 AsHSP26.8 负调控植物的生长和发育，并在植物对多种非生物胁迫的反应中发挥不同的作用。
