Gautam Ranjana, Meena Rajesh Kumar, Rampuria Sakshi, Shukla Pawan, Kirti P B
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, India.
Department of Life Sciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, Uttar Pradesh, India.
Front Plant Sci. 2023 Apr 19;14:1135552. doi: 10.3389/fpls.2023.1135552. eCollection 2023.
Reduced crop productivity results from altered plant physiological processes caused by dysfunctional proteins due to environmental stressors. In this study, a novel DnaJ Type-I encoding gene, having a zinc finger motif in its C-terminal domain was found to be induced early upon treatment with heat stress (within 5 min) in a heat tolerant genotype of RMO-40. is induced by multiple stresses. In tobacco, ectopic expression of reduced ABA sensitivity during seed germination and the early stages of seedling growth of transgenic tobacco plants. Concomitantly, it also improved the ability of transgenic tobacco plants to withstand drought stress by modulating the photosynthetic efficiency, with the transgenic plants having higher F/F ratios and reduced growth inhibition. Additionally, transgenic plants showed a reduced build-up of HO and lower MDA levels and higher chlorophyll content during drought stress, which attenuated cell damage and reduced oxidative damage. An analysis using the qRT-PCR study demonstrated that overexpression is associated with the expression of some ROS-detoxification-related genes and stress-marker genes that are often induced during drought stress responses. These findings suggest a hypothesis whereby positively influences drought stress tolerance and ABA signalling in transgenic tobacco, and suggests that it is a potential gene for genetic improvement of drought and heat stress tolerance in crop plants.
作物生产力下降是由于环境压力导致蛋白质功能失调，进而改变植物生理过程所致。在本研究中，发现一种新型的I型DnaJ编码基因，其C端结构域具有锌指基序，在耐热基因型RMO - 40中，热胁迫处理后早期（5分钟内）即被诱导。该基因受多种胁迫诱导。在烟草中，该基因的异位表达降低了转基因烟草种子萌发和幼苗生长早期对脱落酸（ABA）的敏感性。同时，它还通过调节光合效率提高了转基因烟草植株耐受干旱胁迫的能力，转基因植株具有更高的F/F比值且生长抑制降低。此外，转基因植株在干旱胁迫期间过氧化氢（HO）积累减少、丙二醛（MDA）水平降低且叶绿素含量更高，这减轻了细胞损伤并减少了氧化损伤。使用实时定量聚合酶链反应（qRT-PCR）研究分析表明，该基因的过表达与干旱胁迫响应期间经常被诱导的一些活性氧解毒相关基因和胁迫标记基因的表达有关。这些发现提出了一个假设，即该基因正向影响转基因烟草的干旱胁迫耐受性和ABA信号传导，并表明它是作物植物干旱和热胁迫耐受性遗传改良的潜在基因。
