Linköping University, Department of Biomedical Engineering, Linköping, Sweden.
J Biomed Opt. 2021 Feb;26(2). doi: 10.1117/1.JBO.26.2.026002.
Hemoglobin oxygen saturation and red blood cell (RBC) tissue fraction are important parameters when assessing microvascular status. Functional information can be attained using temporally resolved measurements performed during stimulus-response protocols. Pointwise assessments can currently be conducted with probe-based systems. However, snapshot multispectral imaging (MSI) can be used for spatial-temporal measurements.
To validate if hemoglobin oxygen saturation and RBC tissue fraction can be quantified using a snapshot MSI system and an inverse Monte Carlo algorithm.
Skin tissue measurements from the MSI system were compared to those from a validated probe-based system during arterial and venous occlusion provocation on 24 subjects in the wavelength interval 450 to 650 nm, to evaluate a wide range of hemoglobin oxygen saturation and RBC tissue fraction levels.
Arterial occlusion results show a mean linear regression R2 = 0.958 for hemoglobin oxygen saturation. Comparing relative RBC tissue fraction during venous occlusion results in R2 = 0.925. The MSI system shows larger dynamic changes than the reference system, which might be explained by a deeper sampling including more capacitance vessels.
The snapshot MSI system estimates hemoglobin oxygen saturation and RBC tissue fraction in skin microcirculation showing a high correlation (R2 > 0.9 in most subjects) with those measured by the reference method.
血红蛋白氧饱和度和红细胞（RBC）组织分数是评估微血管状态的重要参数。通过在刺激-反应协议期间进行的时间分辨测量，可以获得功能信息。目前可以使用基于探头的系统进行逐点评估。然而，快照多光谱成像（MSI）可用于时空测量。
验证是否可以使用快照 MSI 系统和反向蒙特卡罗算法来量化血红蛋白氧饱和度和 RBC 组织分数。
在 24 名受试者的波长间隔为 450 至 650nm 的动脉和静脉阻塞激发期间，将来自 MSI 系统的皮肤组织测量值与经过验证的基于探头的系统进行比较，以评估广泛的血红蛋白氧饱和度和 RBC 组织分数水平。
动脉闭塞结果显示血红蛋白氧饱和度的平均线性回归 R2=0.958。比较静脉闭塞期间相对 RBC 组织分数的结果得出 R2=0.925。MSI 系统显示出比参考系统更大的动态变化，这可能是由于更深的采样包括更多的电容血管所致。
快照 MSI 系统估计皮肤微循环中的血红蛋白氧饱和度和 RBC 组织分数，与参考方法测量的结果具有高度相关性（大多数受试者的 R2>0.9）。
