Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai20136, People's Republic of China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai201306, People's Republic of China.
Br J Nutr. 2022 Jan 28;127(2):165-176. doi: 10.1017/S0007114521000544. Epub 2021 Feb 15.
Excessive hepatic glycogen accumulation commonly impairs hepatocytes function and further produces negative effects on growth and health status of carnivorous fish. A 9-week feeding trial was conducted to explore the potential regulation of resveratrol (RSV) on high-carbohydrate-induced glycogen deposition and immune response of largemouth bass. Results showed that high dietary carbohydrate (10 % inclusion of starch) led to hepatic glycogen accumulation and post-prandial hyperglycemia compared with the diet with 5 % starch, which was both alleviated with the inclusion of RSV. The use of RSV promoted the expression of sirtuin 1, which was down-regulated by high dietary carbohydrate. Meanwhile, RSV inclusion promoted the expression of genes involved in insulin pathway and glycolysis and inhibited the expression of gluconeogenesis-related genes. Additionally, high dietary carbohydrate significantly reduced lysozyme content but increased complement C4 content, which were both reversed with RSV supplementation. Meanwhile, RSV inclusion inhibited the expression of pro-inflammatory cytokines but promoted anti-inflammatory cytokines expression, compared with the high carbohydrate treatment. In conclusion, RSV inclusion was beneficial in alleviating high-dietary-carbohydrate-induced glycogen accumulation and immune response in largemouth bass.
过量的肝糖原积累通常会损害肝细胞功能，并对肉食性鱼类的生长和健康状况产生负面影响。进行了为期 9 周的饲养试验，以探讨白藜芦醇（RSV）对高碳水化合物诱导的糖原沉积和大口黑鲈免疫反应的潜在调节作用。结果表明，与含 5%淀粉的饲料相比，高碳水化合物（10%淀粉含量）日粮导致肝糖原积累和餐后高血糖，而添加 RSV 则缓解了这种情况。RSV 的使用促进了沉默信息调节因子 1（sirtuin 1）的表达，而高碳水化合物饮食会下调其表达。同时，RSV 添加促进了胰岛素途径和糖酵解相关基因的表达，并抑制了糖异生相关基因的表达。此外，高碳水化合物饮食显著降低了溶菌酶含量，但增加了补体 C4 含量，而 RSV 补充则逆转了这种情况。同时，与高碳水化合物处理相比，RSV 添加抑制了促炎细胞因子的表达，但促进了抗炎细胞因子的表达。综上所述，RSV 添加有利于缓解大口黑鲈高碳水化合物饮食诱导的糖原积累和免疫反应。
