Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Jinnah Women University, Faculty of Pharmacy, Karachi, Pakistan.
Pak J Pharm Sci. 2020 Jul;33(4):1623-1629.
Aseel is amongst the most vital date variety of Pakistan. Beside its nutritional value, it also got remedial uses therefore for the first time different in-vitro bioassays were performed to assess its medicinal value. Aqueous (DFAE) and ethanol (DFEE) extracts of fresh Aseel dates were used for the purpose. Microplate alamar blue assay was done for antibacterial activity, Brine shrimp lethality test for cytotoxicity and MTT assays with different cancer cell lines were used for anti-cancer activity. Antioxidant and anti-inflammatory activity were also evaluated by free radical scavenging bioassay and chemiluminescence technique. Alamar blue assay of both extracts exhibited weak antibacterial activity against E.coli, S, flexenari and S. aureus. Brine shrimp lethality revealed absence of cytotoxicity at 1000μ/mL concentration. DFEE 50 μ/mL was effective against MCF-7,MDA-MB-231, PC3, 3T3 and Hela cancer cell lines showing 17.59%, 20.90%, 37.60%, 22.35% and 36.70% inhibition whereas DFAE exhibits 20.46%, 30.86%, 15.21%, 29.70% and 16.40 % inhibition respectively. Similarly both extracts also showed varying degree of anti-oxidant and anti-inflammatory activity against standard drug. The results are suggestive of weak bioactivity of Aseel date extracts might because of reduced potency however further studies are required for better understanding of observed results and separation of active ingredients from Aseel dates.
阿塞勒枣是巴基斯坦最重要的枣品种之一。除了营养价值外，它还有药用价值，因此首次进行了不同的体外生物测定来评估其药用价值。本研究使用了新鲜阿塞勒枣的水（DFAE）和乙醇（DFEE）提取物。采用微量板阿尔玛蓝法测定抗菌活性，盐水虾致死试验测定细胞毒性，MTT 法测定不同癌细胞系的抗癌活性。还通过自由基清除生物测定法和化学发光技术评估了抗氧化和抗炎活性。两种提取物的阿尔玛蓝试验均显示出对大肠杆菌、S、flexenari 和金黄色葡萄球菌的弱抗菌活性。在 1000μ/mL 浓度下，盐水虾致死试验未显示出细胞毒性。DFEE 50μ/mL 对 MCF-7、MDA-MB-231、PC3、3T3 和 Hela 癌细胞系有效，抑制率分别为 17.59%、20.90%、37.60%、22.35%和 36.70%，而 DFAE 则分别为 20.46%、30.86%、15.21%、29.70%和 16.40%。同样，两种提取物对标准药物也表现出不同程度的抗氧化和抗炎活性。结果表明，阿塞勒枣提取物的生物活性较弱，可能是由于效力降低，但需要进一步研究以更好地理解观察到的结果并从阿塞勒枣中分离出活性成分。
