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抗炎、抗癌(MCF-7、3T3 和 HeLa 细胞系)以及提取物和全植物馏分的卤虫致死试验和傅里叶变换红外光谱分析。

Anti-Inflammatory, Anticancer (MCF-7, 3T3, and HeLa Cell Lines), and Brine Shrimp Lethality Assay and FTIR Analysis of the Extract and Fractions of the Whole Plant of .

机构信息

Institute of Biochemistry, University of Balochistan, Quetta 87300, Pakistan.

Department of Botany, University of Balochistan, Quetta 87300, Pakistan.

出版信息

Mediators Inflamm. 2020 Feb 1;2020:5056897. doi: 10.1155/2020/5056897. eCollection 2020.

Abstract

In this study, anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies were evaluated. The oxidative burst assay using the chemiluminescence technique, MTT assay, brine shrimp lethality assay, and FTIR analysis were the methods used for the evaluation of anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies, respectively. The whole-plant butanol fraction of (WBFHE) showed anti-inflammatory activity on ROS having IC14.7 ± 2.5 while the extract and other fractions of the whole plant of exhibited no anti-inflammatory activity. None of the extract and fractions of the whole plant of exhibited anticancer (MCF-7, 3T3, and HeLa cell lines) activities. The whole-plant aqueous fraction of (WAFHE) and whole-plant butanol fraction of (WBFHE) showed lethality at high concentration while at low concentration, no toxicity was shown. The whole-plant methanolic extract of (WMEHE) and whole-plant n-hexane fraction of (WHFHE) exhibited no toxicity. FTIR interpretation showed the functional groups for the aromatic compounds, phenols, carboxylic acids, esters, alkanes, alkenes, alcohols, alkyl halides, sulfate esters, phosphines, silanes, nitriles, thiols, amines, phosphoric acids, and nitro compounds.

摘要

在这项研究中,评估了抗炎、抗癌、卤虫致死和 FTIR 研究。使用化学发光技术评估氧化爆发测定法、MTT 测定法、卤虫致死测定法和 FTIR 分析,分别用于抗炎、抗癌、卤虫致死和 FTIR 研究的评估。(WBFHE)的全植物正丁醇级分显示对 ROS 具有抗炎活性,IC14.7±2.5,而全植物提取物和其他级分则没有抗炎活性。全植物的提取物和级分均没有显示出抗癌(MCF-7、3T3 和 HeLa 细胞系)活性。(WAFHE)的全植物水级分和(WBFHE)的全植物正丁醇级分在高浓度时表现出致死性,而在低浓度时则没有显示出毒性。(WMEHE)的全植物甲醇提取物和(WHFHE)的全植物正己烷级分没有显示出毒性。FTIR 解释表明存在芳香族化合物、酚类、羧酸、酯类、烷烃、烯烃、醇类、烷基卤化物、硫酸盐酯类、膦类、硅烷类、腈类、硫醇类、胺类、磷酸和硝基化合物的功能基团。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/000f/7016474/0ac2584742ca/MI2020-5056897.001.jpg

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