Brandenburg D, Gattner H G, Schermutzki W, Schüttler A, Uschkoreit J, Weimann J, Wollmer A
Adv Exp Med Biol. 1977;86A:261-82. doi: 10.1007/978-1-4684-3282-4_16.
Crosslinked insulins have proved to be valuable for structure-function studies and as proinsulin models. In the first part of the paper, a short review of the literature on analytical investigations, the preparation of A1-B1- and A1-B29-crosslinked derivatives, their biological activities in vivo and in vitro, and CD-spectral properties is given. The results of reduction/reoxidation studies with insulin derivatives containing irreversible and cleavable crosslinks are summarized. In the second part, new A1-B29-crosslinked monomers and 3 symmetrical dimers, linked between A1-A'1, B1-B'1 and B29-B'29, are described, as well as some results of tritium-labelling and of enzymatic degradation experiments with A1-B29-linked insulins.
交联胰岛素已被证明在结构-功能研究以及作为胰岛素原模型方面具有重要价值。在本文的第一部分，对有关分析研究、A1-B1-和A1-B29-交联衍生物的制备、它们在体内和体外的生物活性以及圆二色光谱性质的文献进行了简要综述。总结了对含有不可逆和可裂解交联的胰岛素衍生物进行还原/氧化研究的结果。在第二部分中，描述了新的A1-B29-交联单体以及连接在A1-A'1、B1-B'1和B29-B'29之间的3种对称二聚体，以及用A1-B29连接的胰岛素进行氚标记和酶降解实验的一些结果。
