Robinson S M, Lübke K
Hoppe Seylers Z Physiol Chem. 1978 Feb;359(2):225-9. doi: 10.1515/bchm.1978.359.1.225.
The degradation of A1,B29-adipolyinsulin by partial hydrolysis, by disulphide-bond exchange, by chymotrypsin and by a cytosolic enzyme fraction of rat liver was measured and compared with that of A1,B29-diacetylinsulin and insulin. The cross-linked insulin is, in general, more resistant than insulin itself or the diacetylated derivative. However, in the case of enzymatic degradation, A1,B29-diacetylinsulin resembles more the cross-linked derivative than insulin. This provides evidence that the properties of A1,B29-adipoylinsulin could not in all circumstances be attributed to its 'proinsulin-like' structure.
通过部分水解、二硫键交换、胰凝乳蛋白酶以及大鼠肝脏胞质酶组分对A1,B29-己二酰胰岛素的降解情况进行了测定，并与A1,B29-二乙酰胰岛素和胰岛素的降解情况进行了比较。一般来说，交联胰岛素比胰岛素本身或二乙酰化衍生物更具抗性。然而，在酶促降解的情况下，A1,B29-二乙酰胰岛素比胰岛素更类似于交联衍生物。这证明在所有情况下，A1,B29-己二酰胰岛素的特性不能都归因于其“胰岛素原样”结构。
