• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于MoS@Au纳米复合材料发光体和核酸外切酶III驱动的DNA步行器的用于检测Siglec-5的电化学发光适配体传感器。

Electrochemiluminescence aptasensor for Siglec-5 detection based on MoS@Au nanocomposites emitter and exonuclease III-powered DNA walker.

作者信息

Fan Zhenqiang, Yao Bo, Ding Yuedi, Xie Minhao, Zhao Jianfeng, Zhang Kai, Huang Wei

机构信息

NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu, 214063, China.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China.

出版信息

Sens Actuators B Chem. 2021 May 1;334:129592. doi: 10.1016/j.snb.2021.129592. Epub 2021 Feb 8.

DOI:10.1016/j.snb.2021.129592
PMID:33584010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7869706/
Abstract

Lectins are highly specific binding proteins for glycoproteins which widely exist in living organisms, playing a vital role in exploring the biological evolution process, such as cellular proliferation, differentiation, carcinogenesis and apoptosis. Therefore, the content monitoring of lectin becomes particularly significant and urgent in the bioanalytical application. In this work, we fabricated an aptasensor, majorly capitalizing the eminent affinity between sialic acid-binding immunoglobulin (Ig)-like lectin 5 (Siglec-5) and nucleic acids aptamer (K19), with nontoxic MoS@Au nanocomposites as electrochemiluminescence (ECL) emitters based on exonuclease III (Exo III)-powered DNA walker for the bioassays of Siglec-5. The DNA track was constructed on the emitters' surface, providing a reliable platform for the DNA walker's autonomous move. In the assay, the primer DNA in the DNA duplex was replaced by Siglec-5 due to the aptamer interactions and repeatedly released to participate in the movement of the DNA walker, further triggering cascade signal amplification. Finally, our aptasensor indicates significant potential for assays of Siglec-5 with a detection limit of 8.9 pM.

摘要

凝集素是一类对糖蛋白具有高度特异性的结合蛋白，广泛存在于生物体中，在探索生物进化过程（如细胞增殖、分化、癌变和凋亡）中发挥着至关重要的作用。因此，在生物分析应用中，凝集素的含量监测变得尤为重要和迫切。在这项工作中，我们构建了一种适体传感器，主要利用唾液酸结合免疫球蛋白（Ig）样凝集素5（Siglec-5）与核酸适体（K19）之间的卓越亲和力，以无毒的MoS@Au纳米复合材料作为基于核酸外切酶III（Exo III）驱动的DNA步行器的电化学发光（ECL）发射体，用于Siglec-5的生物测定。DNA轨道构建在发射体表面，为DNA步行器的自主移动提供了一个可靠的平台。在测定中，由于适体相互作用，DNA双链中的引物DNA被Siglec-5取代，并反复释放以参与DNA步行器的移动，进一步触发级联信号放大。最后，我们的适体传感器在Siglec-5测定中显示出显著的潜力，检测限为8.9 pM。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/478e3b42f9de/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/99aee5bbbb0a/ga1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/4e7794646847/sc1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/ca5402fc3f25/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/fc767153a256/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/a352a3cefc4f/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/aba06ad46920/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/478e3b42f9de/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/99aee5bbbb0a/ga1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/4e7794646847/sc1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/ca5402fc3f25/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/fc767153a256/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/a352a3cefc4f/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/aba06ad46920/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcd7/7869706/478e3b42f9de/gr5_lrg.jpg

相似文献

1
Electrochemiluminescence aptasensor for Siglec-5 detection based on MoS@Au nanocomposites emitter and exonuclease III-powered DNA walker.基于MoS@Au纳米复合材料发光体和核酸外切酶III驱动的DNA步行器的用于检测Siglec-5的电化学发光适配体传感器。
Sens Actuators B Chem. 2021 May 1;334:129592. doi: 10.1016/j.snb.2021.129592. Epub 2021 Feb 8.
2
MoS Quantum Dots as New Electrochemiluminescence Emitters for Ultrasensitive Bioanalysis of Lipopolysaccharide.基于 MoS 量子点的新型电致化学发光生物传感器用于超灵敏分析脂多糖。
Anal Chem. 2017 Aug 15;89(16):8335-8342. doi: 10.1021/acs.analchem.7b01558. Epub 2017 Jul 27.
3
An electrochemical aptasensor based on intelligent walking DNA nanomachine with cascade signal amplification powered by nuclease for Mucin 1 assay.基于智能行走 DNA 纳米机器的电化学生物传感器，通过核酸酶进行级联信号放大，用于黏蛋白 1 的检测。
Anal Chim Acta. 2022 Jun 29;1214:339964. doi: 10.1016/j.aca.2022.339964. Epub 2022 May 21.
4
An ultrasensitive aptasensor based on self-enhanced Au nanoclusters as highly efficient electrochemiluminescence indicator and multi-site landing DNA walker as signal amplification.一种基于自增强 Au 纳米团簇作为高效电化学发光指示剂和多位点着陆 DNA walker 作为信号放大的超灵敏适体传感器。
Biosens Bioelectron. 2019 Apr 1;130:262-268. doi: 10.1016/j.bios.2019.01.057. Epub 2019 Feb 1.
5
Ultrasensitive detection of PCB77 based on Exonuclease III-powered DNA walking machine.基于 Exonuclease III 驱动的 DNA 行走机器的 PCB77 的超灵敏检测。
J Hazard Mater. 2021 Aug 15;416:125831. doi: 10.1016/j.jhazmat.2021.125831. Epub 2021 Apr 9.
6
Ru(bpy)-Silica@Poly-L-lysine-Au as labels for electrochemiluminescence lysozyme aptasensor based on 3D graphene.Ru(bpy)-Silica@聚-L-赖氨酸-Au 作为标记物的基于 3D 石墨烯的电化学发光溶菌酶适体传感器。
Biosens Bioelectron. 2018 May 30;106:50-56. doi: 10.1016/j.bios.2018.01.059. Epub 2018 Jan 31.
7
A label-free aptasensor for ultrasensitive Pb detection based on electrochemiluminescence resonance energy transfer between carbon nitride nanofibers and Ru(phen).基于氮化碳纳米纤维与 Ru(phen)之间的电致化学发光共振能量转移的无标记适体传感器用于超灵敏 Pb 检测
J Hazard Mater. 2018 Oct 5;359:121-128. doi: 10.1016/j.jhazmat.2018.07.033. Epub 2018 Jul 8.
8
Ultrasensitive sensing platform for platelet-derived growth factor BB detection based on layered molybdenum selenide-graphene composites and Exonuclease III assisted signal amplification.基于层状二硒化钼-石墨烯复合材料和 Exonuclease III 辅助信号放大的血小板衍生生长因子 BB 超灵敏传感平台。
Biosens Bioelectron. 2016 Mar 15;77:69-75. doi: 10.1016/j.bios.2015.09.026. Epub 2015 Sep 12.
9
A triple-amplification SPR electrochemiluminescence assay for chloramphenicol based on polymer enzyme-linked nanotracers and exonuclease-assisted target recycling.基于聚合物酶联纳米示踪物和exonuclease 辅助目标循环的氯霉素三重扩增 SPR 电化学发光分析。
Biosens Bioelectron. 2016 Dec 15;86:477-483. doi: 10.1016/j.bios.2016.07.007. Epub 2016 Jul 5.
10
Interaction of an Iridium(III) Complex with G-Quadruplex DNA and Its Application in Luminescent Switch-On Detection of Siglec-5.一种铱（III）配合物与G-四链体DNA的相互作用及其在唾液酸结合免疫球蛋白样凝集素5的荧光开启检测中的应用
Anal Chem. 2016 Oct 18;88(20):10290-10295. doi: 10.1021/acs.analchem.6b03128. Epub 2016 Oct 6.

引用本文的文献

1
Newly Developed Electrochemiluminescence Based on Bipolar Electrochemistry for Multiplex Biosensing Applications: A Consolidated Review.基于双极电化学的新兴电致化学发光在多重生物传感应用中的研究进展：综述。
Biosensors (Basel). 2023 Jun 19;13(6):666. doi: 10.3390/bios13060666.
2
Recent Trends in Metal Nanoparticles Decorated 2D Materials for Electrochemical Biomarker Detection.金属纳米粒子修饰二维材料在电化学生物标志物检测中的最新进展。
Biosensors (Basel). 2023 Jan 5;13(1):91. doi: 10.3390/bios13010091.
3
DNA Walkers for Biosensing Development.

本文引用的文献

1
Photocontrolled Thermosensitive Electrochemiluminescence Hydrogel for Isocarbophos Detection.光控热敏电化学发光水凝胶用于异恶草酮检测。
Anal Chem. 2020 Apr 21;92(8):6136-6143. doi: 10.1021/acs.analchem.0c00719. Epub 2020 Apr 3.
2
Depolymerization-Induced Electrochemiluminescence of Insoluble Porphyrin in Aqueous Phase.水相中非溶性卟啉的解聚诱导电化学发光。
Anal Chem. 2020 Apr 7;92(7):5464-5472. doi: 10.1021/acs.analchem.0c00208. Epub 2020 Mar 12.
3
Therapeutic options for the 2019 novel coronavirus (2019-nCoV).2019新型冠状病毒（2019-nCoV）的治疗选择。
DNA 行走者在生物传感开发中的应用。
Adv Sci (Weinh). 2022 Jun;9(18):e2200327. doi: 10.1002/advs.202200327. Epub 2022 Apr 22.
4
SARS-CoV-2 monitoring by automated target-driven molecular machine-based engineering.基于自动目标驱动分子机器工程的严重急性呼吸综合征冠状病毒2监测
Environ Chem Lett. 2022;20(4):2227-2233. doi: 10.1007/s10311-022-01434-9. Epub 2022 Apr 12.
5
Molybdenum Disulfide-Based Nanoprobes: Preparation and Sensing Application.基于二硫化钼的纳米探针：制备及传感应用。
Biosensors (Basel). 2022 Jan 31;12(2):87. doi: 10.3390/bios12020087.
6
A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a -activity.一种基于CRISPR/Cas12a活性的用于检测新型冠状病毒核糖核酸聚合酶基因的pH工程再生DNA四面体电化学发光生物传感器。
Chem Eng J. 2022 Feb 1;429:132472. doi: 10.1016/j.cej.2021.132472. Epub 2021 Sep 15.
Nat Rev Drug Discov. 2020 Mar;19(3):149-150. doi: 10.1038/d41573-020-00016-0.
4
Transmission routes of 2019-nCoV and controls in dental practice.2019-nCoV 的传播途径与口腔诊疗中的感染控制
Int J Oral Sci. 2020 Mar 3;12(1):9. doi: 10.1038/s41368-020-0075-9.
5
Determination of the concentration of transcription factor by using exonuclease III-aided amplification and gold nanoparticle mediated fluorescence intensity: A new method for gene transcription related enzyme detection.利用exonuclease III 辅助扩增和金纳米粒子介导的荧光强度测定转录因子浓度：一种用于基因转录相关酶检测的新方法。
Anal Chim Acta. 2020 Apr 1;1104:132-139. doi: 10.1016/j.aca.2019.12.076. Epub 2020 Jan 3.
6
Dual-Wavelength Electrochemiluminescence Ratiometric Biosensor for NF-κB p50 Detection with Dimethylthiodiaminoterephthalate Fluorophore and Self-Assembled DNA Tetrahedron Nanostructures Probe.基于二甲硫代二氨基对苯二甲酸荧光团和自组装 DNA 四面体纳米结构探针的 NF-κB p50 双波长电化学发光比率型生物传感器
ACS Appl Mater Interfaces. 2020 Mar 11;12(10):11409-11418. doi: 10.1021/acsami.0c01243. Epub 2020 Mar 2.
7
Novel coronavirus takes flight from bats?新型冠状病毒从蝙蝠传播？
Nat Rev Microbiol. 2020 Apr;18(4):191. doi: 10.1038/s41579-020-0336-9.
8
Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro.瑞德西韦和氯喹在体外能有效抑制新出现的新型冠状病毒（2019 - 新冠病毒）。
Cell Res. 2020 Mar;30(3):269-271. doi: 10.1038/s41422-020-0282-0. Epub 2020 Feb 4.
9
A pneumonia outbreak associated with a new coronavirus of probable bat origin.一种新型冠状病毒引发的肺炎疫情，该病毒可能来源于蝙蝠。
Nature. 2020 Mar;579(7798):270-273. doi: 10.1038/s41586-020-2012-7. Epub 2020 Feb 3.
10
Matrix Coordination-Induced Electrochemiluminescence Enhancement of Tetraphenylethylene-Based Hafnium Metal-Organic Framework: An Electrochemiluminescence Chromophore for Ultrasensitive Electrochemiluminescence Sensor Construction.基质协调诱导的基于四苯乙烯的铪金属有机骨架的电致化学发光增强：用于超灵敏电致化学发光传感器构建的电致化学发光生色团。
Anal Chem. 2020 Feb 18;92(4):3380-3387. doi: 10.1021/acs.analchem.9b05444. Epub 2020 Jan 30.