NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, Jiangsu, China.
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, P. R. China.
ACS Appl Mater Interfaces. 2020 Mar 11;12(10):11409-11418. doi: 10.1021/acsami.0c01243. Epub 2020 Mar 2.
In this work, we fabricated a dual-wavelength electrochemiluminescence ratiometric biosensor based on electrochemiluminescent resonance energy transfer (ECL-RET). In this biosensor, Au nanoparticle-loaded graphitic phase carbon nitride (Au-g-CN) as a donor and Au-modified dimethylthiodiaminoterephthalate (TAT) analogue (Au@TAT) as an acceptor were investigated for the first time. Besides, tetrahedron DNA probe was immobilized onto Au-g-CN to improve the binding efficiency of the transcription factor and ECL ratiometric changes on the basis of the ratio of ECL intensities at 595 and 460 nm, which were obtained through the formation of a sandwich structure of DNA probe-antigen-antibody. Our biosensor achieved the assay of NF-κB p50 with a detection limit of 5.8 pM as well as high stability and specificity.
在这项工作中，我们基于电化学发光共振能量转移（ECL-RET）构建了一种双通道电化学发光比率生物传感器。在该生物传感器中，首次研究了负载金纳米粒子的石墨相氮化碳（Au-g-CN）作为供体和金修饰的二甲硫二氨基对苯二甲酸酯（TAT）类似物（Au@TAT）作为受体。此外，四面体 DNA 探针固定在 Au-g-CN 上，以提高转录因子的结合效率，并根据通过 DNA 探针-抗原-抗体三明治结构形成获得的 595nm 和 460nm 处的电化学发光强度比值进行电化学发光比率变化。我们的生物传感器实现了 NF-κB p50 的检测，检测限低至 5.8pM，具有高稳定性和特异性。
