Nicot Florence, Dimeglio Chloé, Migueres Marion, Jeanne Nicolas, Latour Justine, Abravanel Florence, Ranger Noémie, Harter Agnès, Dubois Martine, Lameiras Sonia, Baulande Sylvain, Chapuy-Regaud Sabine, Kamar Nassim, Lhomme Sébastien, Izopet Jacques
CHU de Toulouse, Hôpital Purpan, Laboratoire de Virologie, Centre National de Référence du Virus de l'Hépatite E, Toulouse, France.
INSERM, U1043, Toulouse, France.
Front Microbiol. 2021 Jan 28;11:634430. doi: 10.3389/fmicb.2020.634430. eCollection 2020.
Hepatitis E virus (HEV) genotype 3 is the most common genotype linked to HEV infections in Europe and America. Three major clades (HEV-3.1, HEV-3.2, and HEV-3.3) have been identified but the overlaps between intra-subtype and inter-subtype p-distances make subtype classification inconsistent. Reference sequences have been proposed to facilitate communication between researchers and new putative subtypes have been identified recently. We have used the full or near full-length HEV-3 genome sequences available in the Genbank database (April 2020; = 503) and distance analyses of clades HEV-3.1 and HEV-3.2 to determine a p-distance cut-off (0.093 nt substitutions/site) in order to define subtypes. This could help to harmonize HEV-3 genotyping, facilitate molecular epidemiology studies and investigations of the biological and clinical differences between HEV-3 subtypes.
戊型肝炎病毒（HEV）基因型3是欧美地区与戊型肝炎病毒感染相关的最常见基因型。已确定了三个主要分支（HEV-3.1、HEV-3.2和HEV-3.3），但亚型内和亚型间p距离的重叠使得亚型分类不一致。已提出参考序列以促进研究人员之间的交流，并且最近发现了新的假定亚型。我们使用了Genbank数据库（2020年4月；n = 503）中可用的全长或接近全长的HEV-3基因组序列，以及对HEV-3.1和HEV-3.2分支的距离分析，以确定p距离截止值（0.093个核苷酸替换/位点）来定义亚型。这有助于统一HEV-3基因分型，促进分子流行病学研究以及对HEV-3亚型之间生物学和临床差异的调查。
