Stephan Lisa, Jakoby Marc, Hülskamp Martin
Botanical Institute, Biocenter, Cologne University, Cologne, Germany.
Front Plant Sci. 2021 Jan 7;11:596065. doi: 10.3389/fpls.2020.596065. eCollection 2020.
Beige and Chediak Higashi (BEACH) domain proteins mediate membrane-dependent processes in eukaryotic cells. The plant BEACH domain protein SPIRRIG in (AtSPI) was shown to display a similar molecular behavior as its yeast and animal homologs, along with a range of cell morphological defects. In addition, AtSPI was shown to interact with the P-body component DCP1, to differentially effect RNA levels and to be involved in the regulation of RNA stability in the context of salt stress responses. To determine, whether the dual function of SPI in apparently unrelated molecular pathways and traits is evolutionary conserved, we analyzed three alleles in . We show that the molecular behavior of the SPI protein and the role in cell morphogenesis and salt stress response are similar in the two species, though we observed distinct deviations in the phenotypic spectrum.
米色和切迪亚克-东综合征（BEACH）结构域蛋白介导真核细胞中依赖膜的过程。植物BEACH结构域蛋白螺旋蛋白（AtSPI）被证明与其酵母和动物同源物表现出相似的分子行为，同时还存在一系列细胞形态缺陷。此外，AtSPI被证明与P小体成分DCP1相互作用，以差异方式影响RNA水平，并参与盐胁迫响应背景下的RNA稳定性调节。为了确定SPI在明显不相关的分子途径和性状中的双重功能是否在进化上保守，我们分析了三个等位基因。我们表明，SPI蛋白的分子行为以及在细胞形态发生和盐胁迫响应中的作用在这两个物种中相似，尽管我们在表型谱中观察到明显的差异。
