Besharati S, Sadeghi A, Ahmadi F, Tajeddin E, Mohammad Salehi R, Fani F, Pouladfar Gh, Nikmanesh B, Majidpour A, Soleymanzadeh Moghadam S, Mirab Samiee S, Rahnamaye Farzami M, Rahbar M, Eslami P, Rakhshani N, Eshrati B, Gouya M M, Fallah F, Karimi A, Owlia P, Alebouyeh M
MSc in Microbiology, Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
MSc Student in Microbiology, Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Iran J Vet Res. 2020 Fall;21(4):269-278.
is considered as a main cause of community-acquired diarrhea in humans, however, sources of the multi-drug resistant (MDR) strains and their link with the disease are not well known.
This study aimed to investigate the frequency, serogroup diversity, and antimicrobial susceptibility patterns of strains in poultry meat and stool samples of patients with community acquired diarrhea in Tehran.
We compared the frequency of non-typhoidal serogroups, the similarities of their resistance patterns to 10 antimicrobial compounds, the prevalence of extended spectrum β-lactamase (ESBL) and ampicillinase C (AmpC) genetic determinants, and class 1 and 2 integrons in 100 chicken meat and 400 stool samples of symptomatic patients in Tehran during June 2018 to March 2019.
was isolated from 75% and 5.5% of the chicken meats and human stool samples, respectively. The chicken meat isolates mainly belonged to serogroup C (88%, 66/75), while the human stool isolates were mainly related to serogroup D (59.1%, 13/22). The MDR phenotype and the most common rates of resistance to antibiotics, including tetracycline, trimethoprim/sulfamethoxazole (TS) and azithromycin, were detected in 4.5% and 45.3%, 59% and 13.6%, 43% and 9.1%, 42% and 9.1% of the human stool and chicken meat samples, respectively. Carriage of , , and genes in the meat isolate with ESBL resistance phenotype and , , and among the 7 meat strains with AmpC resistance phenotype was not confirmed using polymerase chain reaction (PCR). High prevalence of class 1 and 2 integrons was characterized and showed a correlation with resistance to TS and chloramphenicol.
These findings showed a lack of association between chicken meats and human isolates due to discrepancy between the characterized serogroups and resistance phenotypes.
被认为是人类社区获得性腹泻的主要病因，然而，多重耐药（MDR）菌株的来源及其与该疾病的联系尚不清楚。
本研究旨在调查德黑兰社区获得性腹泻患者家禽肉和粪便样本中菌株的频率、血清群多样性及抗菌药物敏感性模式。
我们比较了2018年6月至2019年3月期间德黑兰100份鸡肉和400份有症状患者粪便样本中非伤寒血清群的频率、其对10种抗菌化合物耐药模式的相似性、超广谱β-内酰胺酶（ESBL）和AmpCβ-内酰胺酶（AmpC）基因决定簇的流行率以及1类和2类整合子。
分别从75%的鸡肉和5.5%的人类粪便样本中分离出。鸡肉分离株主要属于C血清群（88%，66/75），而人类粪便分离株主要与D血清群相关（59.1%，13/22）。在4.5%和45.3%、59%和13.6%、43%和9.1%、42%和9.1%的人类粪便和鸡肉样本中分别检测到MDR表型以及对四环素、甲氧苄啶/磺胺甲恶唑（TS）和阿奇霉素等抗生素的最常见耐药率。使用聚合酶链反应（PCR）未确认具有ESBL耐药表型的肉分离株中、和基因的携带情况以及7株具有AmpC耐药表型的肉菌株中的、和基因。1类和2类整合子的高流行率得到了表征，并显示出与对TS和氯霉素耐药的相关性。
这些发现表明，由于特征性血清群和耐药表型之间存在差异，鸡肉和人类分离株之间缺乏关联。
