Hamaguchi Y, Morizono T, Juhn S K
Department of Otolaryngology, University of Minnesota School of Medicine, Minneapolis.
Am J Otolaryngol. 1988 Jan-Feb;9(1):34-40. doi: 10.1016/s0196-0709(88)80006-1.
The round window membrane (RWM) permeability to human serum albumin (HSA) was investigated in both normal chinchillas and chinchillas sensitized with HSA. The effect of a corticosteroid agent (triamcinolone) on the RWM permeability was also analyzed. It was found that HSA could not be detected in either the perilymph or the cerebrospinal fluid of normal chinchillas within 1 hour after instillation into the middle ear bulla. Perilymph levels of HSA peaked 24 hours after instillation. In antigen-induced otitis media, the HSA level in the perilymph at 12 hours after challenge was significantly higher than that in normal chinchillas (P less than .01), but it did not increase with the development of otitis media. A significant difference of HSA level in the perilymph of animals with and without steroid treatment was noted (P less than .025 at 24 hours and P less than .05 at 48 hours). It is concluded that little HSA passes through the normal RWM in a short time and that the RWM permeability to HSA increases in the early stage (about 12 hours) of antigen-induced otitis media. Corticosteroids can affect the RWM permeability to HSA by reducing the level of antigen-induced otitis media.
研究了正常龙猫和用人类血清白蛋白（HSA）致敏的龙猫的圆窗膜（RWM）对HSA的通透性。还分析了一种皮质类固醇药物（曲安奈德）对RWM通透性的影响。结果发现，将HSA滴入中耳鼓泡后1小时内，正常龙猫的外淋巴或脑脊液中均未检测到HSA。HSA的外淋巴水平在滴入后24小时达到峰值。在抗原诱导的中耳炎中，激发后12小时外淋巴中的HSA水平显著高于正常龙猫（P小于0.01），但它并未随着中耳炎的发展而增加。观察到接受和未接受类固醇治疗的动物外淋巴中HSA水平存在显著差异（24小时时P小于0.025，48小时时P小于0.05）。结论是，短时间内很少有HSA通过正常的RWM，并且在抗原诱导的中耳炎早期（约12小时）RWM对HSA的通透性增加。皮质类固醇可通过降低抗原诱导的中耳炎水平来影响RWM对HSA的通透性。
