Pan Hong, Shi Hongyan, Fu Peng, Shi Pengfei, Yang Jianyuan
Department of Thyroid and Breast Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, 26 Shengli Street, Wuhan 430014, China.
ACS Omega. 2021 Jan 25;6(5):3991-3998. doi: 10.1021/acsomega.0c05924. eCollection 2021 Feb 9.
Cancer vaccine is well recognized as a promising approach for immunotherapy of cancers. Since dendritic cells (DCs) are capable of processing and presenting antigens to initiate the immune response cascade, the development of DC vaccines is considered as a good choice for the treatment of cancer. Herein, a folic acid (FA)-modified liposome was constructed and loaded with chlorin e6 (Ce6) as a DC vaccine (FA-Lipo-Ce6). It was suggested that the loaded Ce6 within FA-Lipo-Ce6 can be activated under laser irradiation. The photodynamic therapy (PDT) of Ce6 was expected to create on-demand reactive oxygen species (ROS) in situ, which causes cell death and trigger the exposure of tumor-associated antigen (TAA). In addition, the produced ROS can mimic the inflammatory responses for the employment of DC for better antigen presentation and immune response. Most importantly, the employment of DC can recognize the exposed TAA to stimulate DC for effective vaccination in situ. Our results demonstrated the powerful capacity of FA-Lipo-Ce6 to induce DC activation, leading to effective suppression of the growth of breast cancers.
癌症疫苗被公认为是一种很有前景的癌症免疫治疗方法。由于树突状细胞（DCs）能够处理和呈递抗原以启动免疫反应级联，因此DC疫苗的开发被认为是治疗癌症的一个不错选择。在此，构建了一种叶酸（FA）修饰的脂质体，并负载二氢卟吩e6（Ce6）作为DC疫苗（FA-Lipo-Ce6）。研究表明，FA-Lipo-Ce6中负载的Ce6在激光照射下可被激活。预计Ce6的光动力疗法（PDT）能原位产生按需活性氧（ROS），导致细胞死亡并引发肿瘤相关抗原（TAA）的暴露。此外，产生的ROS可模拟炎症反应，以利用DC进行更好的抗原呈递和免疫反应。最重要的是，利用DC可识别暴露的TAA，从而刺激DC进行原位有效疫苗接种。我们的结果证明了FA-Lipo-Ce6诱导DC激活的强大能力，从而有效抑制乳腺癌的生长。
