鸡蛋花乳胶提取物蛋白对小鼠乙醇诱导胃损伤的保护作用

Protective effect of proteins extracted from Plumeria pudica latex on ethanol-induced gastric injury in mice.

作者信息

Moita Lucas Arruda, Souza Bruna da Silva, Oliveira Naylla Veras de Moraes, Sales Ana Clara Silva, Oliveira Lucas Eduardo Silva, Oliveira Ana Patrícia de, Sousa Francisca Beatriz Melo, Medeiros Jand-Venes Rolim, Oliveira Jefferson Soares de

机构信息

Universidade Federal do Delta do Parnaíba - Laboratório de Bioquímica de Plantas Laticíferas - Parnaíba (PI), Brazil.

Universidade Federal do Delta do Parnaíba - Laboratório de Inflamação e Gastroenterologia Translacional - Parnaíba (PI), Brazil.

出版信息

Acta Cir Bras. 2025 Jun 6;40:e404025. doi: 10.1590/acb404025. eCollection 2025.

DOI:10.1590/acb404025

PMID:40498952

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12143448/

Abstract

PURPOSE

To evaluate proteins from Plumeria pudica latex (LPPp) for their protective effect against ethanol-induced gastric injury in mice.

METHODS

The LPPp fraction was obtained by collecting P. pudica latex in tubes containing distilled water, followed by centrifugation and dialysis. The female Swiss mice (Mus musculus) received saline or LPPp (40 mg/kg) intraperitoneally 1 hour before oral administration of 500 μL of 50% ethanol. One hour later, the animals were euthanized, and their stomachs were removed for evaluation of tissue lesion area, histopathological analysis, and measurements of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and nitrate/nitrite (NO3/NO2). An independent experiment assessed the effect of LPPp on gastric mucus production.

RESULTS

The LPPp-treated animals showed a significant reduction in the mean injured areas of gastric tissue (0.73 ± 1.01 mm2) compared to the ethanol group (37.99 ± 3.11 mm2). Histopathological analysis revealed significant preservation of tissue architecture in the LPPp group compared to ethanol group. Additionally, LPPp maintained tissue levels of MDA, GSH, SOD, and NO3/NO2 comparable to the saline group and influenced gastric mucus production favorably (p < 0.05).

CONCLUSION

These results suggested that LPPp has a protective effect against ethanol-induced gastric lesions, likely involving antioxidant activity and increased gastric mucus production.

摘要

目的

评估鸡蛋花乳胶蛋白（LPPp）对小鼠乙醇诱导的胃损伤的保护作用。

方法

通过将鸡蛋花乳胶收集到含有蒸馏水的试管中，然后进行离心和透析来获得LPPp组分。雌性瑞士小鼠（小家鼠）在口服500μL 50%乙醇前1小时腹腔注射生理盐水或LPPp（40mg/kg）。1小时后，对动物实施安乐死，并取出它们的胃以评估组织损伤面积、进行组织病理学分析以及测量丙二醛（MDA）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和硝酸盐/亚硝酸盐（NO3/NO2）。一项独立实验评估了LPPp对胃黏液产生的影响。

结果

与乙醇组（37.99±3.11mm2）相比，LPPp处理的动物胃组织平均损伤面积显著减少（0.73±1.01mm2）。组织病理学分析显示，与乙醇组相比，LPPp组的组织结构得到显著保存。此外，LPPp使MDA、GSH、SOD和NO3/NO2的组织水平维持在与生理盐水组相当的水平，并对胃黏液产生有有利影响（p<0.05）。