Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, P. R. China.
J Agric Food Chem. 2021 Feb 24;69(7):2236-2244. doi: 10.1021/acs.jafc.0c06084. Epub 2021 Feb 15.
Volatile terpenoids are a large group of important secondary metabolites and possess many biological activities. The acyclic sesquiterpene -nerolidol is one of the typical representatives and widely used in cosmetics and agriculture. Here, the accumulation of volatile terpenes in different tissues of was investigated, and two -nerolidol synthases, CaNES1 and CaNES2, were identified and characterized by enzymatic assays. Both genes are differentially transcribed in different tissues of . Next, we constructed a cell factory to enable high-level production of -nerolidol. Glucose was the sole carbon source to sequentially control gene expression between the competitive squalene and -nerolidol pathways. Finally, the -nerolidol production of recombinant strain LWG003- was 7.01 g/L by fed-batch fermentation in a 5 L bioreactor. The results clarify volatile terpenoid biosynthesis in and provide a promising potential for industrial production of -nerolidol in .
挥发性萜类化合物是一大类重要的次生代谢产物，具有许多生物活性。无环倍半萜 - 香叶醇是其中的典型代表之一，广泛应用于化妆品和农业。在这里，我们研究了不同组织中挥发性萜类化合物的积累情况，并通过酶促反应鉴定和表征了两种 - 香叶醇合酶，CaNES1 和 CaNES2。这两个基因在 的不同组织中差异转录。接下来，我们构建了一个 细胞工厂，以实现 - 香叶醇的高水平生产。葡萄糖是唯一的碳源，用于在竞争的角鲨烯和 - 香叶醇途径之间顺序控制基因表达。最后，通过在 5 L 生物反应器中进行分批补料发酵，重组菌株 LWG003- 的 - 香叶醇产量达到 7.01 g/L。这些结果阐明了 在挥发性萜类化合物生物合成中的作用，并为 在工业生产 - 香叶醇方面提供了有前景的潜力。
