Characterization of -Nerolidol Synthase from Maxim and Production of -Nerolidol in Engineered .

Volatile terpenoids are a large group of important secondary metabolites and possess many biological activities. The acyclic sesquiterpene -nerolidol is one of the typical representatives and widely used in cosmetics and agriculture. Here, the accumulation of volatile terpenes in different tissues of was investigated, and two -nerolidol synthases, CaNES1 and CaNES2, were identified and characterized by enzymatic assays. Both genes are differentially transcribed in different tissues of . Next, we constructed a cell factory to enable high-level production of -nerolidol. Glucose was the sole carbon source to sequentially control gene expression between the competitive squalene and -nerolidol pathways. Finally, the -nerolidol production of recombinant strain LWG003- was 7.01 g/L by fed-batch fermentation in a 5 L bioreactor. The results clarify volatile terpenoid biosynthesis in and provide a promising potential for industrial production of -nerolidol in .