• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码 RNA 9708-1 在阴道假丝酵母菌病小鼠模型中的抗真菌作用。

Antifungal Effect of Long Noncoding RNA 9708-1 in the Vulvovaginal Candidiasis Murine Model.

机构信息

Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, 210004, Jiangsu, People's Republic of China.

The Second Affiliated Hospital of Medical University of Anhui, Hefei, 230601, Anhui, People's Republic of China.

出版信息

Mycopathologia. 2021 May;186(2):177-188. doi: 10.1007/s11046-021-00530-8. Epub 2021 Feb 15.

DOI:10.1007/s11046-021-00530-8
PMID:33587236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8106589/
Abstract

Vulvovaginal candidiasis (VVC) caused by Candida spp. affects 70-75% of women at least once during their lives. We aim to elucidate the potential mechanism of VVC and investigate the therapeutic effects of long noncoding RNA 9708-1. Female BALB/c mice were randomized to four treatment groups, including the blank control group, VVC control group, vehicle control group and lncRNA 9708-1-overexpressed group. Mice were euthanized on Day 4, Day 7 and Day 14 after treatment. Colony-forming unit (CFU) was measured, and the inflammation was detected by hematoxylin and eosin (H&E). Gene and protein expression levels of lncRNA 9708-1 and FAK were determined by real-time PCR, Western blot and immunohistochemistry. The overexpression of lncRNA 9708-1 significantly decreased the fungal load from Day 4 to 7. H&E staining indicated that the impaired histological profiles were improved in lncRNA 9708-1-overexpressed group. LncRNA 9708-1 led to a significant increase in FAK level of vagina tissue which is expressed mainly in epithelial basal layer. This study suggests that lncRNA 9708-1 played a protective role on murine experimental VVC by upregulating the expression levels of FAK.

摘要

阴道假丝酵母菌病（VVC）由假丝酵母菌属引起，影响 70-75%的女性在其一生中至少发作一次。我们旨在阐明 VVC 的潜在机制，并研究长链非编码 RNA 9708-1 的治疗效果。雌性 BALB/c 小鼠被随机分为四组治疗组，包括空白对照组、VVC 对照组、载剂对照组和 lncRNA 9708-1 过表达组。治疗后第 4、7 和 14 天处死小鼠。测量集落形成单位（CFU），并通过苏木精和伊红（H&E）染色检测炎症。通过实时 PCR、Western blot 和免疫组化测定 lncRNA 9708-1 和 FAK 的基因和蛋白表达水平。lncRNA 9708-1 的过表达从第 4 天到第 7 天显著降低真菌负荷。H&E 染色表明，lncRNA 9708-1 过表达组中受损的组织学特征得到改善。lncRNA 9708-1 导致阴道组织中 FAK 水平显著增加，FAK 主要表达在上皮基底细胞层。这项研究表明，lncRNA 9708-1 通过上调 FAK 的表达水平对实验性小鼠 VVC 发挥保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/0e2a8d5edff8/11046_2021_530_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/925f7818be0a/11046_2021_530_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/20975983aa39/11046_2021_530_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/226c34f73bf8/11046_2021_530_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/cdd7a8398fe6/11046_2021_530_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/0e2a8d5edff8/11046_2021_530_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/925f7818be0a/11046_2021_530_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/20975983aa39/11046_2021_530_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/226c34f73bf8/11046_2021_530_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/cdd7a8398fe6/11046_2021_530_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4448/8106589/0e2a8d5edff8/11046_2021_530_Fig5_HTML.jpg

相似文献

1
Antifungal Effect of Long Noncoding RNA 9708-1 in the Vulvovaginal Candidiasis Murine Model.长链非编码 RNA 9708-1 在阴道假丝酵母菌病小鼠模型中的抗真菌作用。
Mycopathologia. 2021 May;186(2):177-188. doi: 10.1007/s11046-021-00530-8. Epub 2021 Feb 15.
2
Pulsatilla decoction inhibits Candida albicans proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the Dectin-1 signaling pathway.白头翁汤通过 Dectin-1 信号通路抑制白色念珠菌在阴道念珠菌病小鼠模型中的增殖和黏附。
J Ethnopharmacol. 2018 Sep 15;223:51-62. doi: 10.1016/j.jep.2018.05.018. Epub 2018 May 26.
3
[Effect of butyl alcohol extract of Baitouweng Decoction on epithelial barrier of vaginal mucosa in mice with vulvovaginal candidiasis].白头翁汤丁醇提取物对外阴阴道假丝酵母菌病小鼠阴道黏膜上皮屏障的影响
Zhongguo Zhong Yao Za Zhi. 2020 Oct;45(20):4991-4996. doi: 10.19540/j.cnki.cjcmm.20200618.401.
4
[Mechanism of n-butanol alcohol extract of Baitouweng Decoction in treatment of vulvovaginal candidiasis based on negative regulation of NLRP3 inflammasome via PKCδ/NLRC4/IL-1Ra axis].[基于PKCδ/NLRC4/IL-1Ra轴对NLRP3炎性小体的负调控探讨白头翁汤正丁醇提取物治疗外阴阴道假丝酵母菌病的机制]
Zhongguo Zhong Yao Za Zhi. 2023 Mar;48(6):1578-1588. doi: 10.19540/j.cnki.cjcmm.20221110.701.
5
vaginitis in a murine model is reduced by polypeptide-enriched extracts.多肽富集提取物可减少小鼠模型中的阴道炎。
Future Microbiol. 2019 Jul;14:839-846. doi: 10.2217/fmb-2018-0299. Epub 2019 Aug 2.
6
[Pulsatilla decoction inhibits vulvovaginal Candida albicans proliferation and reduces inflammatory cytokine levels in vulvovaginal candidiasis mice].白头翁汤抑制外阴阴道念珠菌病小鼠模型中白色念珠菌的增殖并降低炎性细胞因子水平
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Feb;32(2):153-7.
7
Efficacy of Histatin5 in a murine model of vulvovaginal candidiasis caused by Candida albicans.Histatin5 在白色念珠菌引起的外阴阴道念珠菌病小鼠模型中的疗效。
Pathog Dis. 2017 Aug 31;75(6). doi: 10.1093/femspd/ftx072.
8
Investigation of thiosemicarbazide free or within chitosan nanoparticles in a murine model of vulvovaginal candidiasis.在阴道假丝酵母菌病的小鼠模型中研究硫代卡巴肼或壳聚糖纳米粒子。
Braz J Microbiol. 2020 Dec;51(4):1465-1473. doi: 10.1007/s42770-020-00326-w. Epub 2020 Jul 8.
9
Prevalence of vulvovaginal candidiasis among pregnant women in the Ho municipality, Ghana: species identification and antifungal susceptibility of Candida isolates.加纳霍市孕妇外阴阴道假丝酵母菌病的流行情况：假丝酵母菌分离株的种属鉴定和抗真菌药敏试验。
BMC Pregnancy Childbirth. 2020 May 6;20(1):266. doi: 10.1186/s12884-020-02963-3.
10
[INVESTIGATION ON ANTIFUNGAL SUSCEPTIBILITY OF CANDIDA YEASTS IN PREGNANT PATIENTS WITH CONFIRMED VULVOVAGINAL CANDIDIASIS AND THEIR NEWBORNS.].[确诊外阴阴道念珠菌病的孕妇及其新生儿念珠菌酵母菌的抗真菌药敏性调查。]
Akush Ginekol (Sofiia). 2016;55(4):20-29.

引用本文的文献

1
LncRNA: A Potential Target for Host-Directed Therapy of Infection.长链非编码RNA：感染宿主导向疗法的潜在靶点。
Pharmaceutics. 2022 Mar 11;14(3):621. doi: 10.3390/pharmaceutics14030621.

本文引用的文献

1
LncRNA FOXC2-AS1 enhances FOXC2 mRNA stability to promote colorectal cancer progression via activation of Ca-FAK signal pathway.长链非编码 RNA FOXC2-AS1 通过激活 Ca-FAK 信号通路增强 FOXC2 mRNA 的稳定性，从而促进结直肠癌的进展。
Cell Death Dis. 2020 Jun 8;11(6):434. doi: 10.1038/s41419-020-2633-7.
2
The Role of 17β-Estrogen in Candida albicans Adhesion on Human Vaginal Epithelial Cells via FAK Phosphorylation.17β-雌二醇通过 FAK 磷酸化在白色念珠菌黏附于阴道上皮细胞中的作用。
Mycopathologia. 2020 Jun;185(3):425-438. doi: 10.1007/s11046-020-00440-1. Epub 2020 Mar 17.
3
Long Noncoding RNA FAM225A Promotes Nasopharyngeal Carcinoma Tumorigenesis and Metastasis by Acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3.
长链非编码 RNA FAM225A 通过作为 ceRNA 吸附 miR-590-3p/miR-1275 并上调 ITGB3 促进鼻咽癌肿瘤发生和转移。
Cancer Res. 2019 Sep 15;79(18):4612-4626. doi: 10.1158/0008-5472.CAN-19-0799. Epub 2019 Jul 22.
4
Association of lnc-IL17RA-11 with increased radiation sensitivity and improved prognosis of HPV-positive HNSCC.长链非编码RNA-IL17RA-11与HPV阳性头颈部鳞状细胞癌放疗敏感性增加及预后改善的相关性
J Cell Biochem. 2019 Oct;120(10):17438-17448. doi: 10.1002/jcb.29008. Epub 2019 May 22.
5
Long Noncoding RNAs in Host-Pathogen Interactions.长非编码 RNA 在宿主-病原体相互作用中的作用。
Trends Immunol. 2019 Jun;40(6):492-510. doi: 10.1016/j.it.2019.04.001. Epub 2019 Apr 30.
6
Identification of lncRNA-155 encoded by MIR155HG as a novel regulator of innate immunity against influenza A virus infection.鉴定由MIR155HG编码的lncRNA - 155作为抗甲型流感病毒感染天然免疫的新型调节因子。
Cell Microbiol. 2019 Aug;21(8):e13036. doi: 10.1111/cmi.13036. Epub 2019 May 29.
7
Candida albicans: An Emerging Yeast Model to Study Eukaryotic Genome Plasticity.白色念珠菌：一种用于研究真核基因组可塑性的新兴酵母模型。
Trends Genet. 2019 Apr;35(4):292-307. doi: 10.1016/j.tig.2019.01.005. Epub 2019 Feb 28.
8
Biofilms and vulvovaginal candidiasis.生物膜与外阴阴道假丝酵母菌病。
Colloids Surf B Biointerfaces. 2019 Feb 1;174:110-125. doi: 10.1016/j.colsurfb.2018.11.011. Epub 2018 Nov 7.
9
Inhibition of CXCR4 by MicroRNA-1192 Reduces the Activation of Th17 Cells and Expression of Inflammation Factors in a Mouse Model of Vulvovaginal Candidiasis.微小RNA-1192对CXCR4的抑制作用可降低外阴阴道念珠菌病小鼠模型中Th17细胞的活化及炎症因子的表达。
Cell Physiol Biochem. 2018;50(3):893-910. doi: 10.1159/000494475. Epub 2018 Oct 24.
10
Safety and Efficacy of a Novel Vaginal Anti-infective, TOL-463, in the Treatment of Bacterial Vaginosis and Vulvovaginal Candidiasis: A Randomized, Single-blind, Phase 2, Controlled Trial.新型阴道抗感染药物 TOL-463 治疗细菌性阴道病和外阴阴道念珠菌病的安全性和疗效：一项随机、单盲、2 期、对照试验。
Clin Infect Dis. 2019 Feb 15;68(5):803-809. doi: 10.1093/cid/ciy554.