Glomeruli from ischemic rat kidneys produce increased amounts of platelet activating factor.

The production of Platelet Activating Factor (PAF-acether) by glomeruli isolated from rats subjected to a 60 min renal ischemia has been studied. PAF-acether has been purified by Sep-Pak columns and measured by its ability to release serotonin from previously loaded rabbit platelets. Glomeruli from ischemic kidneys had higher amounts of PAF-acether activity than glomeruli from sham operated rats. These data suggest a role for PAF-acether in the renal functional alterations induced by renal ischemia.