血小板醚连接甘油磷酸胆碱中血小板活化因子乙酰醚的形成及花生四烯酸的释放。

Paf-acether formation and arachidonic acid freeing from platelet ether-linked glyceryl-phosphorylcholine.

作者信息

Chignard M, Le Couedic J P, Coëffier E, Benveniste J

出版信息

Biochem Biophys Res Commun. 1984 Oct 30;124(2):637-43. doi: 10.1016/0006-291x(84)91602-4.

Abstract

Ether-linked glyceryl-phosphorylcholines (GPC) are potential precursors for paf-acether biosynthesis. We show that such phospholipids are present in rabbit platelets in sufficient amounts to insure lyso paf-acether and paf-acether formation. Indeed, upon stimulation, platelets formed lyso paf-acether and paf-acether in amounts representing 10% and 0.2% of the total amounts of ether-linked GPC. Following preincubation with [3H]paf-acether, ether-linked GPC labelled at position 1 of the glycerol were detected in platelets. They were hydrolysed, and gave birth to lyso paf-acether upon platelet activation. Platelet ether-linked GPC labelled at position 2 with [3H]arachidonic acid (AA) were also hydrolyzed under the same experimental conditions. These data indicate that in rabbit platelets these phospholipids are precursors for paf-acether and AA, two mediators of platelet activation.

摘要

醚连接的甘油磷酰胆碱（GPC）是血小板活化因子（PAF）生物合成的潜在前体。我们发现，此类磷脂在兔血小板中的含量足以确保溶血PAF和PAF的形成。事实上，受到刺激后，血小板形成的溶血PAF和PAF的量分别占醚连接GPC总量的10%和0.2%。用[3H]PAF预孵育后，在血小板中检测到甘油1位标记的醚连接GPC。它们被水解，并在血小板活化时产生溶血PAF。在相同实验条件下，用[3H]花生四烯酸（AA）标记甘油2位的血小板醚连接GPC也被水解。这些数据表明，在兔血小板中，这些磷脂是PAF和AA的前体，而PAF和AA是血小板活化的两种介质。