Hansen T B
MBG, Aarhus University, C.F. Moellers Alle 3, 8000 Aarhus C, Denmark.
Methods. 2021 Dec;196:68-73. doi: 10.1016/j.ymeth.2021.02.007. Epub 2021 Feb 13.
Within recent years, circular RNAs (circRNAs) have been an attractive new field of research in RNA biology and disease. Consequently, numerous studies have been published towards the disclosure of circRNA biogenesis and function. Initially, circRNAs were described as a subclass of cytoplasmic non-coding RNA, however, a few recent observations have proposed that circRNAs may instead be templates for protein production. The extent to which this is the case is currently debated, and therefore using rigorous data analysis and proper experimental setups is instrumental to settle the current controversies. Here, the conventional experiments used for detecting circRNA translation are outlined, and guidelines to distinguish signal from the inherent noise are discussed. While these guidelines are specific for circRNA translation, most also apply to other aspects of non-canonical translation.
近年来，环状RNA（circRNA）已成为RNA生物学和疾病研究中一个引人关注的新领域。因此，已经发表了大量关于揭示circRNA生物合成和功能的研究。最初，circRNA被描述为细胞质非编码RNA的一个亚类，然而，最近的一些观察结果表明，circRNA可能反而作为蛋白质产生的模板。目前，这种情况的程度仍存在争议，因此使用严格的数据分析和适当的实验设置有助于解决当前的争议。在这里，概述了用于检测circRNA翻译的传统实验，并讨论了区分信号与固有噪声的指导原则。虽然这些指导原则是针对circRNA翻译的，但大多数也适用于非经典翻译的其他方面。
