Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.
Center for Cellular Signal Patterns (CellPAT), Aarhus University, Aarhus, Denmark.
Nat Methods. 2022 Oct;19(10):1208-1220. doi: 10.1038/s41592-022-01487-2. Epub 2022 May 26.
Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors' experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.
环状 RNA（circRNAs）存在于所有生命领域，并通过不同的机制产生。近年来，环状 RNA 相关研究论文数量呈爆炸式增长；然而，作为一个相对较年轻的领域，环状 RNA 生物学迫切需要用于环状 RNA 分离、分析、表达和耗尽的通用实验标准。在这里，我们根据作者的经验提出了一套环状 RNA 研究指南。本观点将特别针对由剪接体催化的反向剪接事件产生的真核生物中主要的一类 circRNAs。我们希望环状 RNA 研究最佳实践原则的实施将有助于推动该领域的发展，并使人们能够更好地理解这组令人着迷的 RNA。
