Lucas D, Ménez J F, Bodénez P, Baccino E, Bardou L G, Floch H H
Laboratoire de Biochimie, UA CNRS 598, Faculté de Médecine, Brest, France.
Alcohol Alcohol. 1988;23(1):23-31.
Acetaldehyde, the first metabolite of ethanol, reacts with haemoglobin in vitro to produce acetaldehyde-haemoglobin adducts. Some clinical studies on the minor haemoglobins have suggested that these adducts may be formed in people abusing alcohol. Under hydrolysis of haemoglobin, with oxalic acid at 100 degrees C in sealed vials, some acetaldehyde was released and then specifically determined by HPLC. The kinetics of hydrolysis were studied using haemoglobin previously labelled with 14[C] acetaldehyde. The maximum liberation of 14 [C] acetaldehyde was obtained after 3 hr 30 min hydrolysis and this time factor was then utilized in the analysis of alcoholic and control haemoglobin. Thus, we have confirmed the formation of acetaldehyde haemoglobin adducts in vivo. It must be noted that the released acetaldehyde corresponds only to an index of the stable adducts. The levels were higher in alcoholics than in controls (1.417 +/- 0.171 and 1.295 +/- 0.139 nmol/mg Hb, respectively, P less than 0.001). In conclusion, this marker is not a convenient tool for the monitoring of alcohol exposure levels because of the low differences between alcoholic and control haemoglobins.
乙醛是乙醇的首个代谢产物，在体外可与血红蛋白反应生成乙醛 - 血红蛋白加合物。一些针对微量血红蛋白的临床研究表明，酗酒者体内可能会形成这些加合物。在密封小瓶中，于100℃下用草酸对血红蛋白进行水解，会释放出一些乙醛，然后通过高效液相色谱法进行特异性测定。使用预先用14[C]乙醛标记的血红蛋白研究水解动力学。水解3小时30分钟后，14[C]乙醛的释放量达到最大值，随后将该时间因素用于分析酗酒者和对照者的血红蛋白。因此，我们证实了体内乙醛 - 血红蛋白加合物的形成。必须指出的是，释放出的乙醛仅对应稳定加合物的一个指标。酗酒者体内的水平高于对照组（分别为1.417±0.171和1.295±0.139 nmol/mg Hb，P＜0.001）。总之，由于酗酒者和对照者血红蛋白之间差异较小，该标志物并非监测酒精暴露水平的便捷工具。
